摘要
目的:建立雷公藤总萜片中雷公藤内酯醇含量的HPLC测定方法。方法:采用Lichrospher CN色谱柱,甲醇 水为流动相,梯度洗脱,检测波长 255 nm。结果:雷公藤内酯醇在 0 742~59 36μg·mL-1线性关系良好,r=0.999 8,平均加样回收率为99.2%(n=5),RSD 1.7%。结论:方法简便快捷,无干扰,重复性好,可用于雷公藤总萜片的质量控制。
Objective: To esdevelop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets. Method:A Lichrospher CN column was used with wethanol and water for gradient elution. The detection wavelength was set at 255 nm. Result: The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 μg·mL^(-1) (r=0.999 8). The average recovery was 99.2%, RSD%=1.7%. Conclusion: The method is simple, rapid and accurate and can be used for quality control of the tablets
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第4期266-267,共2页
China Journal of Chinese Materia Medica
