期刊文献+

骨髓间充质干细胞对异基因骨髓移植后GVHD和生存率的影响 被引量:8

The Effect of Bone Marrow Mesenchymal Stem Cells on Graft Versus Host Disease and Survival Rate After Allogeneic Bone Marrow Transplantation
原文传递
导出
摘要 目的 观察骨髓间充质干细胞 (BMMSC)对急性淋巴细胞白血病 (ALL)小鼠异基因骨髓移植 (allo -BMT)后移植物抗宿主病 (GVHD)和生存率的影响。方法 建立ALL小鼠动物模型 ,对其进行allo -BMT的同时 ,静脉输注体外培养的供鼠BMMSC ,同时设立单纯allo -BMT对照组。流式细胞仪检测受鼠CD4+ 、CD8+ T细胞亚群的差异 ;观察受鼠发生GVHD一般反应及病理学变化 ;记录受鼠存活时间。结果 BMMSC减少受鼠CD4+ T细胞的同时增加CD8+ T细胞 ;推迟GVHD发生的时间 ;明显延长ALL小鼠allo -BMT后的生存时间 (P <0 0 5或P <0 0 1)。结论 BMMSC能抑制ALL小鼠allo -BMT后GVHD的发生 ,但同时具有一定程度的GVL作用。 Objective To observe the effect of bone marrow mesenchymal stem cells (BMMSC) on graft versus host disease (GVHD) and survival rate after allogenic bone marrow transplantation (allo-BMT) in acute lymphocytic leukemia (ALL) mice. Methods In the experimental group, both bone marrow cells and BMMSC obtained from donor mice were transplanted into the recipient mice injected with leukemia cells five days ago, and in the control group, the mice was injected with bone marrow cells alone. The impact of BMMSC on the quantity of CD 4 + and CD 8 + T cells after allo-BMT was evaluated by flow cytometry. The general manifestation and pathological changes of GVHD were observed. The survival time of recipient mice was recorded. Results BMMSC could decrease the quantity of CD 4 + T cells, increase CD 8 + T cells number, delay GVHD, and obviously increase the survival time in the mice treated with allo-BMT(P<0.05 or P<0.01). Conclusion BMMSC could alleviate GVHD and maintain graft versus leukimia (GVL) after allo-BMT in mice.
出处 《中国医师杂志》 CAS 2005年第2期219-221,共3页 Journal of Chinese Physician
关键词 GVHD BMT 异基因骨髓移植 生存率 骨髓间充质干细胞 小鼠 CD8^+T细胞 目的观 结论 差异 Bone marrow mesenchymal stem cells Bone marrow transplantation Graft versus host disease Graft versus leukemia
  • 相关文献

参考文献5

  • 1Dormady SP, Bashayan O, Dougherty R, et al. Immortalized multipotential mesenchymal cells and the hematopoietic microenvironment[J]. Hematother Stem Cell Res, 2001, 10:125-140
  • 2Noort WA, Kruisselbrink AB, Anker PS, et al. Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34+cells in NOD/SCID mice[J]. Exp Hematol, 2002, 30: 870-878
  • 3Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo[J]. Exp Hematol, 2002, 30:42-48
  • 4Le Blanc K, Tammik C, Sundberg B, et al. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex[J]. Scand J Immunol, 2003, 57:11-20
  • 5岑建农,陈子兴,钱军,王玮,傅建新,潘金兰.造血干细胞移植预处理对人骨髓间充质干细胞的影响[J].江苏医药,2004,30(4):259-260. 被引量:8

二级参考文献3

  • 1Sukhikh GT,Malaitsev VV,Bogdanova IM,et al.Mesenchymal stem cells.Bulletin of Exp Biology and Medicine,2002,133 :103-109.
  • 2Maria A,Enrico N,Joanna EG,et al.Cotransplantation of human mesenchymal stem cells enhances human myelopoiesis and megakaryocytopoiesis in NOD/SCID mice.Exp Hematol,2003,31:413-420.
  • 3Jiang Y,Jahagirdar BN,Reinhardt R,et al.Pluripotency of meseenchymal stem cells derived from adult marrow.Nature,2002,20:1-9.

共引文献7

同被引文献89

引证文献8

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部