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建立快速检测鼠疫耶尔森菌的胶体金免疫层析法 被引量:14

The Establishment of Gold-immunochromatography for Fast Detection of Yersinia Pestis of Bubonic Plague
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摘要 〔目的〕 建立检测鼠疫耶尔森菌 (鼠疫杆菌 )的胶体金标记免疫层析方法。〔方法〕 采用柠檬酸三钠还原法制备胶体金颗粒 ,并标记鼠疫杆菌的抗体 ,制成免疫层析检测试纸条 ,进行特异性和敏感性评价 ,并对面粉、土壤、奶粉、淀粉等不同材料进行模拟添加鼠疫菌检测。〔结果〕 用多种不同的常见细菌或近缘细菌进行测试 ,未出现非特异性反应 ,检测鼠疫杆菌的灵敏度为 1× 10 5cfu/ml,对水、土壤、奶粉、面粉、淀粉、酵母粉等不同材料中鼠疫杆菌进行检测也取得同样结果 ,10~ 15min即可得到结果。〔结论〕 建立了 1种可以用于现场快速检测鼠疫杆菌的胶体金免疫层析方法 ,为进一步大规模应用奠定了基础。
出处 《中国国境卫生检疫杂志》 CAS 2004年第6期332-335,共4页 Chinese Journal of Frontier Health and Quarantine
基金 国家质量监督检验检疫总局重大科研基金项目
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