摘要
为了解益生菌保健食品中益生菌的耐药性 ,采用E Test方法 ,对中国益生菌保健品市场上常用的菌株进行耐药检测。所用抗生素为抑制细菌细胞壁、细菌核酸合成和蛋白质合成的 13种耐药实验常用抗生素 (阿莫西林 可克拉维、万古霉素、复方新诺明、甲氧苄胺嘧啶、庆大霉素、氯霉素、链霉素、四环素、丁胺卡那霉素、卡那霉素、萘啶酮酸、头孢曲松和头孢噻吩 )。结果表明 :在检测的12株益生菌中 ,除动物双歧杆菌FDBb 12耐受 2种抗生素外 ,其余菌株分别耐受 3～ 9种抗生素 ,属于多重耐药菌。耐药的主要模式为丁胺卡那霉素 (12 12 )、卡那霉素 (12 12 )、萘啶酮酸 (11 12 )、复方新诺明 (10 12 )、甲氧苄胺嘧啶 (9 12 )和万古霉素 (7 12 )。其中 ,罗伊氏乳杆菌的耐药性最强 ,对 13种抗生素中的 9种耐药。建立并加强国内益生菌的安全评价和耐药性监测体系是必要而迫切的。
Antibiotic susceptibility testings were made by gradient diffusion (E Test; AB Biodisk) on deMan, Rogosa, Sharpe (MRS) agar plate for 12 strains of probiotics commonly used as an ingredient of functional foods. The antibiotics included, Amoxicillin/clavulanic acid, Vancomycin, Trimethoprin/sulfamethoxazone, Trimethoprin, Gentamicin, Chloramphenicol, Streptomyxin, Tetracycline, Amikacin, Kanamycin, Nalidixic acid, Ceftriaxone, and Cephalothin. The result showed that all strains were resistant to Amikacin and Kanamycin, and sensitive to Amoxicillin/clavulanic acid and Chloramphenicol. The main resistant pattern in antibiotic susceptibility testing was Amikacin(12/12), Kanamycin(12/12), Nalidixic acid (11/12), Trimethoprin/sulfamethoxazone (10/12), Trimethoprin(9/12)and Vancomycine(7/12).
出处
《中国食品卫生杂志》
2005年第2期108-112,共5页
Chinese Journal of Food Hygiene
