摘要
胚胎植入和胎盘形成涉及细胞外基质的降解和重建,以及细胞的增殖、凋亡、迁移和分化,基质金属蛋白酶(MMPs) 是参与这些事件的主要蛋白水解酶系统. MMP-26是近年来发现的MMPs家族的新成员,但其功能所知甚少. 通过半定量RT-PCR、免疫组织化学、荧光免疫细胞化学等手段,发现人胎盘中MMP-26主要定位于绒毛滋养层细胞,在绒毛间质细胞中也有少量表达. 妊娠早期,胎盘中MMP-26表达水平较高,至妊娠中期降至最低,但在足月胎盘中其表达又有显著提高,提示MMP-26可能参与妊娠早期滋养层细胞的侵润和分娩时的胎盘剥离. 体外培养的妊娠早期人细胞滋养层细胞能产生一定水平的MMP-26,而其表达受到激活素A的剂量依赖性刺激,表明滋养层细胞中存在MMP-26表达的自分泌/旁分泌调节.
The processes of implantation and placentation involve various cell events, such as degradation and remodeling of extracellular matrix, cellular proliferation, apoptosis, and differentiation. Evidence indicates that members of the matrix metalloproteinase (MMP) family play crucial roles in these processes. In the present study, the expression and localization of MMP-26/endometase/matrilysin-2 in human cytotrophoblast cells as well as its regulation by activin A were investigated by using methods of reverse transcriptase-polymerase chain reaction, immunohischemistry and fluorescence immunohistochemistry. The results showed that MMP-26 was strongly localized in villous trophoblast cells, and weakly in some of the villous mesenchyma. The mRNA level of MMP-26 in chorionic villi was high in early pregnancy, and was down-regulated from gestational week 10 on, reaching a very low level at week 26. However, its mRNA expression was significantly up-regulated in the term placenta. In the primary cultured cytotrophoblast cells, the mRNA expression of MMP-26 maintained a stable level during gestational week 6similar to12. Moreover, the expression of MMP-26 in the cytotrophoblast cells was stimulated by activin A in a dose-dependent manner. All the data indicated that MMP-26 may be involved in trophoblast invasion during early pregnancy and placenta detachment at the time of delivery. There may exist autocrine/paracrine regulation of MMP-26 expression in cytotrophoblast cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第1期25-30,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(30370542)
国家重点基础发展专项项目(G1999055903)
中国科学院知识创新重要方向项目(KSCX-2-SW-201).~~
