摘要
目的 观察体外定向诱导大鼠骨髓基质细胞分化为脂肪细胞和成骨细胞。方法 全骨髓法分离大鼠骨髓基质细胞 ,传 3代后分别在成脂、成骨诱导条件下继续培养 ,油红O染色、碱性磷酸酶染色和VonKossa染色判定其分化结果。结果 传 3代大鼠骨髓基质细胞成脂诱导 2 1d后 ,分化为脂肪细胞的阳性率为 (83. 6± 2 . 8) % ,成骨诱导 12d后碱性磷酸酶染色阳性率达 (87. 6± 2. 8) % ,连续诱导35d可见矿化结节形成。结论 随着诱导条件的不同 ,大鼠骨髓基质细胞在体外可定向分化为脂肪细胞或成骨细胞。
Objective To investigate the differentiation from bone marrow stromal cells to adipocytes and osteoblasts. Methods Bone marrow stromal cells were isolated from both femora in Wistar rats using all-bone marrow culture method. The cells were subcultured, collected after passage 3, and induced to adipocytes or osteoblasts by adipogenic inducer or osteogenic inducer. The differentiation of adipocytes or osteoblasts from bone marrow stromal cells was observed by oil red O, alkaline phosphatase and Von Kossa staining. Results After 21 days of adipocyte inducing, about (83.6±2.8)% of the cells displayed accumulation of lipid vacuoles. The rate of alkaline phosphatase positive cells reached (87.6±2.8)% after 12 days of osteoblast inducing. When cultured up to 35 days, there was calcium deposition in the nodules. Conclusions Under appropriate conditions, bone marrow stromal cells can be induced to differentiate along the adipocytic or osteoblastic pathways.
出处
《中国骨质疏松杂志》
CAS
CSCD
2005年第1期45-48,共4页
Chinese Journal of Osteoporosis
