摘要
目的观察饮用含不同质量浓度和不同形态的无机砷(iAsⅢ,iAsⅤ)水,砷化物在小鼠肝和脑组织中的分布及代谢情况。方法小鼠以自由饮水方式暴露iAsⅢ或iAsⅤ42d,采用氢化物发生-超低温捕集-原子吸收分光光度法,测定小鼠肝和脑组织中无机砷(iAs)、一甲基胂(MA)、二甲基胂(DMA)和三甲基胂(TMA)水平。结果在小鼠肝组织中,iAs、MA及DMA均随染砷量的增加而升高;在脑组织中,iAs在各染砷组与对照组之间,差异无统计学意义。DMA在各染砷组中随染砷剂量的增加而升高,但脑组织中未检测到MA。结论经饮水进入小鼠体内的iAsⅢ或iAsⅤ,主要在肝组织内进行甲基化代谢,低质量浓度的iAsⅢ较iAsⅤ更容易进入肝组织,并被甲基化代谢。砷化物可以透过成鼠的血脑屏障,脑组织分布的砷化物形态以DMA为主。
Objective To observe the distribution and metabolism of arsenic speciation in the brain and liver of mice exposed to different concentrations of inorganic AsⅢ or AsV through drinking water. Methods Mice were exposed to As by drinking water for 42 days. Tissues of liver and brain was taken to assessed the contents of inorganic arsenic (iAs), monomethylarsenic acid(MMA),dimethylarsenic acid(DMA) and trimethylarsenic compound (TMA) hydride generation trapping by ultra-hypothermia coupled with atomic absorption spectrometry. Results Contents of iAs,MA and DMA in liver increased along with the iAs concentrations in drinking water. In brain,contents of DMA increased along with the arsenic concentration in drinking water. MA was not found in brain in both control and exposed mice. Conclusions iAs taken from drinking water is mainly distributed and methylated in the liver. AsⅢ of a low level enters the liver more easily comparing with AsV. Arsenicals enter brain through the brain-blood barrier of adult mice, and DMA, as a main arsenide, distributes in the brain.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2005年第2期137-139,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金资助项目(30400362)
