摘要
目的探讨性激素水平对染氟大鼠生精细胞凋亡的调节作用。方法通过腹腔注射氟化钠(NaF)溶液的方法建立大鼠氟中毒模型,采用放射免疫的方法检测血清睾酮和雌二醇水平,采用末端标记法(TUNEL)检测凋亡的生精细胞。结果①NaF10、20mg·kg-1·(2d)-1染毒28d时,睾酮水平为(10.39±0.54)、(8.34±1.29)nmol/L,较对照组降低(P<0.05或0.01);染毒38d时,睾酮水平为(9.82±0.66)、(6.33±0.68)nmol/L,较对照组降低(P<0.01)。②NaF10、20mg·kg-1·(2d)-1染毒28d时,雌二醇水平为(44.24±11.32)、(36.27±3.00)pmol/L,较对照组降低(P<0.01);染毒38d时,雌二醇水平为(42.90±9.81)、(26.02±16.14)pmol/L,较对照组降低(P<0.01)。③与对照组相比,各染氟组生精细胞凋亡率均升高(P<0.01)。④生精细胞凋亡率与血清睾酮水平呈负相关(r值为-0.901～-0.886),与血清雌二醇水平呈负相关(r值为-0.989～-0.936)。结论氟在一定剂量和作用时间内可致大鼠血清性激素水平紊乱,是导致生精细胞凋亡的重要原因之一。
Objective To observe the regulating effect of sex hormone levels on spermatogenic cell apoptosis in male rats exposed to fluoride. Methods The fluorosis model was set up by peritoneal injection of NaF solution. The serum hormone levels were radioimmunochemically detected, and the apoptotic spermatogenic cells were quantitatively measured by TUNEL. Results ① Compared with control rats, administration of 10 and 20 mg·kg-1·(2 d)-1 NaF for 28 days could decrease serum testosterone level to(10.39±0.54),(8.34±1.29) nmol/L (P < 0.05 or 0.01), respectively,the latter being(9.82±0.66),(6.33±0.68) nmol/L on day 38 (P < 0.01), respectively. ② Compared with control rats, administration of 10 and 20 mg·kg-1·(2 d)-1 NaF for 28 days could decrease serum estradiol level to (44.24±11.32), (36.27±3.00) pmol/L (P < 0.01), respectively, the later were lower, being (42.90±9.81),(26.02±16.14) pmol/L on day 38(P < 0.01), respectively. ③ Compared with control rats, the apoptotic spermatogenic cell ratio of fluoride treatment group was significantly increased (P < 0.01). ④ The apoptotic spermatogenic cell ratio was negatively correlated with serum testosterone level (r = - 0.901～- 0.886) and serum estradiol level (r = - 0.989～- 0.936). Conclusions Excessive fluoride results in disturbance of serum hormone levels, which is an important cause for spermatogenic cell apoptosis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2005年第2期149-151,共3页
Chinese Jouranl of Endemiology
