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上样量和洗脱液温度影响磁珠法纯化mRNA效果 被引量：1

Influence of Loading Amount and Eluant Temperature to Effect of mRNA Purification by Magnetic Particles
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摘要 对总RNA上样量和洗脱液温度的比较试验表明,用Promega公司生产的磁珠法试剂盒PolyAT-tractmRNAIsolationSystemsⅢZ5300纯化mRNA,每管总RNA上样量以550滋g适中。将洗脱液加热至R70℃,有利于提高洗脱率。550滋g上样量与70℃洗脱液温度配合运用,既可保证mRNA纯化质量,充分利用总RNA样品,又可避免磁珠浪费。 Comparative experiment among different loading amount and eluant temperature showed that the optimal loading amount for magneticsphere mRNA purification kit produced by Promega (PolyA TtractR mRNA Isolation Systems Ⅲ Z5300) was about 550μg. Heating the eluant to 70℃ was beneficial to raising elution ratio. Using loading amount of 550 μg together with eluant temperature of 70℃ not only ensures the quality of mRNA purification and utilizes total RNA sample, but also avoids the waste of magnetic particles.
作者 倪娜 李晚忱
机构地区 四川农业大学玉米研究所
出处 《分子植物育种》 CAS CSCD 2005年第2期282-284,共3页 Molecular Plant Breeding
关键词 磁珠法 洗脱液 总RNA 纯化 效果 配合 试剂盒 比较试验 温度 利用 Loading amount,Eluant temperature,Magnetic particle,mRNA purification
分类号 Q7-33 [生物学—分子生物学]
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参考文献4

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  • 1樊昕,王刚,刘玉峰.寻常型银屑病患者趋化因子受体7表达及其意义探讨[J].中国皮肤性病学杂志,2006,20(12):724-726. 被引量：6
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分子植物育种
2005年 第2期

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