摘要
目的 探讨缺氧缺血脑损伤对新生大鼠HIBD脑皮质Fas-L蛋白表达的影响,及清开灵对新生大鼠 HIBD的干预治疗作用以及有关分子机制。方法 建立HIBD动物模型后免疫组化测定,分为清开灵干预组、生 理盐水对照组、假手术组,根据检测需要,在缺氧缺血后24小时、3天、7天作Fas-L蛋白免疫组化SP法测定。结 果 左脑皮质Fas-L蛋白表达阳性率假手术组、生理盐水组、清开灵干预组:24小时依次为8.04±3.50,120.20± 6.10,21.10±5.10;3天依次为8.20±3.10,87.6±5.90,13.40±4.20;7天依次为8.01±2.80,8.32±2.90,8.26±2.83;生理盐水 组与另两组分别比较,7天P均>0.05,无明显变化;24.小时、3天增多明显(P<0.05),清开灵干预组降低明显(P< 0.05)。结论 新生大鼠HIBD后,结扎侧脑皮质Fas-L蛋白表达增高。清开灵能抑制新生大鼠HIBD结扎侧脑皮 质Fas-L蛋白的高表达。
Objective To explore the expression of fas-L protein of cortex in newborn rats HIBD. To explore the effects of QingKaiLing on them. Methods Animals were divided into sham group, NS group and QingKaiLing group. The changes of expression of fas-L protein were detected at 24h, 3d and 7d after hypoxia ischemia by using immunohistochemistry. Results Comparing with the sham group, the expression of fas-L protein (3d and 7d) increased obviously in the ligated side of the brain cortex in NS group (P<0.05). Comparing with NS group, they were on the contrary in QingKaiLing group (P<0.05). Conclusions The expression of fasL protein became higher rapidly after HIBD. QingKaiLing can refrain the expression of fas-L protein.
出处
《海南医学》
CAS
2005年第4期129-130,共2页
Hainan Medical Journal
基金
铁道部科研基金资助课题
项目编号 BS-98058。
关键词
脑缺氧
脑缺血
新生大鼠
清开灵
FAS-L
Cerebral-hypoxia
Cerebral-ischemia
Neonatal rat
QingKaiLing
fas-L
