摘要
根据人MHC-DRB和MHC-DQB基因组序列和猪MHC-DRB和MHC-DQB基因外显子1设计引物,应 用PCR扩增及克隆测序技术,首次得到了猪MHC-DRB和MHC-DQB基因的5'上游近端调控区(URR)序列。分 析发现所得序列中存在与MHCⅡ类基因表达调控有关的高度保守的W、X、Y、CCAAT及类TATA调控元件,调控 元件的空间组织顺序也与其他物种相应序列的相同。利用SSCP技术在313头猪中共发现12个DRB-URR复等 位基因和14个DQB-URR复等位基因,序列比对结果表明在这些复等位基因中存在丰富的多态位点,为进一步深 入研究猪MHCⅡ类基因近端调控区的多态性及抗病育种研究奠定了基础。
The primers,RF/RR and QF1/QR,were designed based on the homologous region of swine MHC-DQB and DRB exon1 sequences and HLA-DQB and HLA-DRB sequences.The 5′ proximal promoter region (URR) nucleotide sequences of swine MHC-DQB and DRB were firstly obtained by PCR,cloning and sequencing techniques.There exist highly conserved sequence motifs including W,X,Y,CCAAT,and TATA-like boxes in the obtained sequences,showing the same organization of the conserved regulatory elements as in other species.12 DRB-URR and 14 DQB-URR alleles were found by PCR-SSCP in 313 pigs.The aligned results of these alleles showed that there exist abundant polymorphism sites in both Porcine MHC-DQB and DRB proximal promoter region.
基金
国家重点基础研究规划项目资助项目(编号:G2000016104)~~
