摘要
采用不连续缓冲系统的聚丙烯酰胺凝胶电泳法（ＰＡＧＥ法），结合组织特异性染色和酶谱区带活性扫描技术，对家鸡胚胎期和生后期不同生长发育阶段各组织器官的乳酸脱氢酶（ＬＤＨ）同工酶进行了测定。结果表明，鸡胚胎期Ｂ型ＬＤＨ同工酶占优势（５１．１～９９．８９％），生后期则有３种不同表现：心、眼、脑组织继续保持Ｂ亚基的优势地位；肝、肾组织呈现Ｂ型酶含量的微弱上升：胸肌则发生Ｂ型酶到Ａ型酶的颠倒转换。ＬＤＨ同工酶的这种表达特征是ｌｄｈａ和ｌｄｈｂ基因实施调控的结果，有利于家鸡各组织在代谢过程中产生足够的能量。
The discontinuous polyacrylamide gel electrophoresis(PAGE)with the tissuespecific staining and zymogram band activity screening techniques was used to detectthe LDH isozymes of the different stages of chick embryo and various tissues and organswith different growth and developmental stages.The result showed that the B-type LDHhad the higher relative value(5l.4～99.8%)in the stage of embryo period.Afterincubation,there were three kinds of expressions for LDH isozymes,i.e.,tbe advantigeous B-type LDH was continuously maintained in heart,ens and brain;the relativevalue of B-type LDH slightly raised in liver and kidney;and the inversion transforma-tion from the B-type LDH to the A type LDH was took place in breast.The expression of LDH isozymes above mentioned is the result of gene regulation by Idh a and Idh b2which has the advantage of producing enough energy in the metabolic process ofchicken,svarious tissues.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1994年第6期513-518,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
