摘要
目的:探讨重症肌无力(myastheniagravis,MG)及MG危象发病机制与下丘脑-垂体-肾上腺(hypothalamus-pituitary-adrenalaxis,HPA)内分泌轴变化的关系,为MG预防性康复措施介入提供依据。方法:本实验于2002-03/2003-10在广西医科大学实验中心完成。实验选用雄性SD大鼠12只,随机分成实验组6只、对照组6只。将乙酰胆碱受体抗体(antinicotinicacetylcholinereceptorantibodies,AchRab)IgG或正常人IgG注入大鼠侧脑室各6只,连续3d,应用原位杂交、放射免疫和化学发光免疫法研究对照组和实验组鼠下丘脑促肾上腺皮质释放激素(corticotropin-releasinghormone,CRH)mRNA转录水平表达和血浆促肾上腺皮质激素(adrenocorticotropichormone,ACTH)及皮质酮水平的变化。结果:在基础状态,实验组下丘脑CRHmRNA阳性细胞数(193.67±13.93)及阳性区面积犤(1099.33±125.70)μm2犦明显低于对照组下丘脑CRHmRNA犤(240.67±18.15),(1303.83±77.46)μm2犦,差异有显著性意义(t=2.932,3.393,P<0.05,0.01)。实验组大鼠ACTH水平犤(56.24±5.57)ng/L犦和皮质酮水平犤(16.13±2.49)nmol/L犦均稍高于对照组犤(54.14±4.04)ng/L,(14.97±1.63)nmol/L犦,但差异均无显著意义(P>0.05)。结论:AchRab能与下丘脑乙酰胆碱受体结合。
AIM: To study the relation between the pathogenesis of myasthenia gravis(MG), and its crisis and the changes of endocritic axis of hypothalamus- pituitary- adrenal(HPA) in order to provide reference for preventative rehabilitation of MG.METHODS: The experiment was finished in Laboratory Center of Guangxi Medical University from March 2002 to October 2003. Twelve male SD rats were randomly divided into experimental group (6 rats) and control group (6 rats). Acetylcholine receptor antibody (AchRab) immunoglobulin G (IgG) or healthy person's IgG was injected into lateral ventricles of rat for 3 consecutive days. The expression of corticotropin- releasing hormone (CRH) mRNA in hypothalamus and the levels of adrenocorticotropic hormone (ACTH) and corticosterone in plasma were observed and quantitatively analyzed by using hybridization in situ and radioimmunoassay and chemiluminescence immunization in both groups.RESULTS: In baseline, the positive cells (193.67± 13.93) and positive- zone [(1 099.33± 125.70) μ m2] of CRHmRNA in hypothalamus in experimental group were significantly lower than those [(240.67± 18.15), (1 303.83± 77.46) μ m2] in control group, and there was significant difference (t=2.932,3.393,P < 0.05, 0.01). The levels of ACTH [(56.24± 5.57) ng/L] and corticosterone [(16.13± 2.49) nmol/L] in experimental group were higher than those[(54.14± 4.04) ng/L,(14.97± 1.63) nmol/L] in control group, but there was no significant difference (P >0.05).CONCLUSION:The results indicate that there is immunological cross- reactivity between AchRab and the neuronal acetylcholine receptor in hypothalamus. AchRab can inhibit the expression of CRHmRNA in hypothalamus in basal state. But it can not affect the ACTH and CORT in two groups. The findings suggest that AchRab may inhibite the activation of HPA in certain conditions,for example, stress. This may be related with pathogenesis of MG and MG crisis.
出处
《中国临床康复》
CSCD
北大核心
2005年第9期57-59,i002,共4页
Chinese Journal of Clinical Rehabilitation
基金
广西科学基金资助项目(桂科基0236024)~~
