摘要
目的:观察大脑中动脉缺血再灌注(MCAO/R)大鼠模型小动脉血管平滑肌细胞(vascularsmoothmusclecells,VSMC)中细丝蛋白的免疫组化表达变化,与平滑肌肌动蛋白表达变化对比,以期了解脑小动脉病变中VSMC的表型转化及细丝蛋白在表型转化中的意义。方法:选取SD大鼠56只制备MCAO/R大鼠模型,依照再灌注时间的不同分为2,6,12,24h,3d及7d共6组,与正常组对照。按时相点取材,免疫组织化学染色,图像分析。结果:α平滑肌肌动蛋白在再灌注6h后表达明显下降,阳性单位(PU)值为17.24±4.36;在24h时表达最低,PU为10.53±2.67,在3d时表达有所升高;7d时表达最高,PU为31.49±1.18,P<0.01;缺血2h后细丝蛋白表达稍有下降,以12h组的表达最低,PU为12.72±1.92,24h组时细丝蛋白表达升高,PU为26.42±1.19,在3d时表达最高,PU为30.12±1.78,P<0.01。结论:再灌注损伤可引起小动脉VSMC表型的变化;细丝蛋白也可作为VSMC表型变化的指标之一,与α平滑肌肌动蛋白相比意义更大;细丝蛋白可能在再灌注中起下调炎症反应及保护细胞的作用。
AIM:To observe the changes of expression of filamin in the arteriolar vascular smooth muscle cell(VSMC) after middle cerebral arteinal occlussion and reperfusion(MCAO/R) so as to find out the meaning of phenotype switching of VSMC and the role of filamin in phenotype switching. METHODS:Fifty- six rats were selected and MCAO/R model was established.The MACO/R group was divided into six groups according to different time points:2 hours,6 hours,12 hours,24 hours,3 days to 7 days and compared with control group.Immunohistochemical staining method and color image analysis were used to observe the expression. RESULTS:Expression of α - smooth muscle- actin decreased after 6- hour reperfusion(PU=17.24± 4.36), and in 24- hour group the expression was the lowest(PU=10.53± 2.67).In 3 days, the expression of actin increased and reached to the top(PU=31.49± 1.18, P < 0.01) in 7 days. Expression of filamin began to decrease after 2- hour reperfusion, and in 12- hour group the expression(PU=12.72± 1.92) was much lower than other groups. In 24 hours group,the expression increased(PU=26.42± 1.19) and in 3 days group was the highest(PU=30.12± 1.78, P< 0.01).CONCLUSION:The reperfusion injury can cause phenotype switching of VSMC.Filamin can be another important sign in phenotype switching of VSMC and may be better than α - smooth muscle- actin.Filamin can relieve inflammatory reaction induced by reperfusion and protect cells.
出处
《中国临床康复》
CSCD
北大核心
2005年第9期78-79,i003,共3页
Chinese Journal of Clinical Rehabilitation
