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骨髓间充质干细胞通过分泌TGF-β1抑制T细胞的增殖 被引量：15

Mesenchymal stem cells inhibit T-lymphocyte proliferation through secreting TGF-β1
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摘要 目的研究是否MSCs通过分泌TGFβ1抑制PHA刺激的T淋巴细胞的增殖。方法应用酶联免疫吸附试验测定MSCs上清中转化生长因子TGFβ1的分泌水平,应用抗TGFβ1抗体中和MSCs上清中的TGFβ1后,用3H掺入法检测在不同培养条件下PHA刺激T淋巴细胞增殖的能力,以及应用Elispot试剂盒检测T淋巴细胞IFNγ的分泌水平。结果MSCs上清减少活化的T淋巴细胞IFNγ的分泌,抑制PHA刺激的T淋巴细胞的增殖能力,且它的这种抑制作用能够被抗TGFβ1抗体部分逆转。结论MSCs通过分泌TGFβ1抑制PHA刺激的T淋巴细胞的增殖。 Objective:To study if MSCs inhibit T-lymphocyte proliferation through secretion of transforming growth factor-beta (TGF-β1).Methods:The level of TGF-β1 was evaluated by enzyme linked immunosorbant assay (ELISA);after the TGF-β1 was neutralized by its correspondig mAb,the ability of T lymphocyte-proliferation was measured by ~~ 3H -Thydimine incorporation;and the level of interferon-gamma (IFN-γ) was measured by Eli-spot kit.Results:MSCs reduced the level of IFN-γ secreted by activated T lymphocytes;inhibited the ability of T-lymphocyte proliferation and such an effect could be reversed partly by anti-TGF-β1 antibody.Conclusion:MSCs inhibit T-lymphocyte proliferation through secreting TGF-β1. [
作者 张伟 葛薇 李长虹 尤胜国 韩钦 邓为民 陈磊 赵春华
机构地区 中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2005年第3期168-171,共4页 Chinese Journal of Immunology
基金 国家自然科学基金资助项目(编号30070284) 国家863课题资助项目(编号2002AA205061)
关键词 骨髓间充质干细胞 转化生长因子 免疫抑制 Bone marrow mesenchymal stem cells TGF-β1 Immune suppression
分类号 R392.11 [医药卫生—免疫学]
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参考文献10

  • 1Pittenger M F,Mackay A M,Beck S C et al.Multilineage potential of adult human mesenchymal stem cells [J].Science,1999;284:143.
  • 2Bartholomew A,Sturgeon C,Siatskas M et al.Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo [J].Exp Hematol,2002;30:42.
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二级参考文献13

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共引文献35

同被引文献148

引证文献15

二级引证文献45

中国免疫学杂志
2005年 第3期

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