期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

TRAIL及其5种受体DNA片段的扩增与序列分析

Preparation of human TRAIL and its five receptors' gene probe
下载PDF
导出
摘要 目的制备TRAIL及其5种受体的特异性基因探针。方法以PHA刺激人外周血淋巴细胞(PBL)增殖,从而诱导TRAIL及其受体的mRNA转录水平提高,然后用RTPCR方法扩增出TRAIL及其5种受体的DNA基因片段,并采用基因测序法确证所得基因片段序列的正确性。结果获得了TRAIL及其5种受体的DNA基因片段。结论所用方法正确,为研究TRAIL及其受体调控细胞凋亡的机制提供了有价值的工具。 Objective:To prepare the probes of human TRAIL and its five receptors.Methods:Human peripheral lymphocytes (PBL) were stimulated to proliferate by PHA. It can induce the gene expression of TRAIL and its receptors at the same time. All coding gene fragments of TRAIL and its five receptors were largely expanded by RT-PCR and isolated by electrophoresis and confirmed by gene sequencing.Results:The DNA fragments of TRAIL and its five receptors were obtained.Conclusion:The adopted method is right. It provides the useful tools for research in the regulating mechanism of the cell apoptosis induced by TRAIL and its receptors. [
作者 季军捷 王华菁 黑振宇 彭玲 王皓 李晓冬 时玉舫 郭亚军
机构地区 上海第二军医大学肿瘤研究所
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2005年第3期213-214,217,共3页 Chinese Journal of Immunology
基金 国家杰出青年科学基金资助项目(39925021)
关键词 肿瘤坏死因子相关凋亡诱导配体 植物血凝素 凋亡 反转录聚合酶链反应 TRAIL PHA Apoptosis RT-PCR
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献9

  • 1Nagata S.Apoptosis by death factor [J].Cell,1997;88(3):355-365.
  • 2Wiley S R,Schooley K,Smolak P G et al.Identification and characterization of a new member of the TNF family that induces apoptosis [J].Immunity,1995;3:673-682.
  • 3Pitti R M,Marsters S A,Ruppert S et al.Induction of apoptosis by Apo-2 ligand,a new member of the TNF cytokine family [J].J Biol Chem,1996;271:12687-12690.
  • 4Jeremias I,Herr I,Boehler T et al.TRAIL/Apo-2 ligand-induced apoptosis in human T cells [J].Eur J Immunol,1998;28:143-152.
  • 5Simonet W S,Laccy D L,Dunstan C R et al.Osteoprotegerin:a novel secreted protein involved in the regulation of bone density [J].Cell,1997;89(2):309-319.
  • 6Emery J G,McDonnell P,Brigham B M et al.Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL [J].J Biol Chem,1998;273(23):14363-14367.
  • 7Ashkenazi A,Dixit V M.Apoptosis control by death and decoy receptors [J].Curr Opin Cell Biol,1999;11(2):255-260.
  • 8Ozawa F,Friess H,Kleeff J et al.Effects and expression of TRAIL and its apoptosis-promoting receptors in human pancreatic cancer [J].Cancer Lett,2001;163(1):71-81.
  • 9Nimmanapalli R,Perkins C L,Orlando M et al.Pretreatment with paclitaxel enhances apo-2 ligand/tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis of prostate cancer cells by inducing death receptors 4 and 5 protein levels [J].Cancer Res,2001;61(2):759-763.
中国免疫学杂志
2005年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部