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羊痘病毒P32蛋白的表达 被引量：8

Expression of Capripoxvirus P32 Protein
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摘要 将已获得的 Bac- Bac1- P32、Bac- Bac HT- P32重组杆状病毒表达载体转染 sf9昆虫细胞 ,得到含羊痘病毒 P32基因的重组杆状病毒 r Bac HT- P32。将 P32基因插入到大肠杆菌表达载体 p ET30 a中 ,转化 BL 2 1感受态细胞 ,IPTG诱导表达。经 SDS-PAGE、Western- blot鉴定 ,在杆状病毒、大肠杆菌中都得到了 35 k Da重组 P32蛋白。 Sf9 cells were respectively transfected by recombinant expression Bacmid: Bac-Bac1-P32、Bac-BacHT-P32, and the recombinant virus contained Capripoxvirus P32 protein gene were obtained. P32 gene was inserted into E.coli expressing vector pET30a and transformed into BL21 cells. The recombiant E.coli was induced by IPTG. The 35kDa recombinant P32 protein obtained in recombinant Baculovirus and E.coli were detected by SDS-PAGE、Western-blot.
作者 郭巍 相文华 李一经
机构地区 中国农业科学院哈尔滨兽医研究所 东北农业大学预防兽医教研室
出处 《中国兽医杂志》 CAS 北大核心 2005年第3期8-10,共3页 Chinese Journal of Veterinary Medicine
关键词 蛋白 大肠杆菌表达 HT 重组杆状病毒 ET 基因插入 转染 羊痘病 SDS-PAGE IPTG Capripoxvirus P32 protein expression Bac-to-Bac^(○R) Baculovirus Expression System pET 30a
分类号 S858 [农业科学—临床兽医学] R581 [医药卫生—内分泌]
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参考文献10

  • 1Distt Nainital, Rao T V, Bandyopadhyay S K.A comprehens-ive reviews of goat pox and sheep pox and their diagnosis[J].Anim Health Res Rev,2000,1(2):127-136.
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  • 4Chand P, Kitching R P,Black D N. Western blot analysis of virus-specific antibody for capripox and contagious pustular dermatitis viral infections in sheep[J]. Epidemiol Infect,1994,113(2):377-385.
  • 5Heine H G, Stevents M P, Foord A J.A capripoxvirus detec-tion PCR and antibody ELISA based on the major P32,the homolog of the vaccinia virus H3L gene[J].J Immunol Methods,1999,227:187-196.
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  • 8郭巍,陈杰,李一经,李晓成,黄保续,张燕霞,吴发兴.羊痘病毒P32蛋白基因的克隆及其杆状病毒表达载体的构建[J].中国预防兽医学报,2004,26(4):270-272. 被引量：9
  • 9郭巍,陈杰,黄保续,李晓成,吴发兴,张燕霞,李一经.应用PCR检测山羊痘病毒[J].中国兽医科技,2003,33(12):50-52. 被引量：29
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二级参考文献12

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  • 6R P Kitching. Studies on the Major Common Precipitation Antigen of Capripoxvirus[ J]. gen Virol. 1986,67:139-148.
  • 7Paul D Gershon. A Comparison of the Genomes of Capripoxvirus Isolates of Sheep, Goat, and Cattle [ J ]. Virology. 1988, 134: 341-349.
  • 8Chand P.Westem blot analysis of virus-specific antibody for capripox and contagious pustular dermatitis viral infections in sheep [ J]. Epidemiol Infect. 1994,113(2): 377-385.
  • 9H G Heine, M P Stevens. A capripoxvirus detection PCR andantibody ELISA based on the major P32, the homolog of the vaccinia virus H3L gene[ J] .J Immunol Methods. 1999,227:187-196.
  • 10V.M. Cam. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus [ J ]. J Virol Methods. 1994,49:285-294.

共引文献34

同被引文献98

引证文献8

二级引证文献67

中国兽医杂志
2005年 第3期

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