摘要
目前螺旋霉素生物合成的发酵杂质含量过高 ,导致成品收率过低 ,杂质含量不符合药典规定。为解决这一问题 ,首先利用LC -MS联用技术定性分析了 4种螺旋霉素发酵杂质的结构 ,结合螺旋霉素生物合成途径分析 ,它们都起因于螺旋霉素生物合成糖基化过程的紊乱。氮源尤其是铵盐对螺旋霉素生物合成糖基化过程有重要的调节作用。进一步的氮源优化使螺旋霉素发酵液中 4种杂质含量降低 2 2 %～ 88% ,杂质含量全部低于欧洲药典标准。
There are several impurities in the spiramycin fermentation broth whic h leads to a lower yield and lower quality of the product. Four impurities in sp iramycin broth have been simultaneously separated and identified by LC-ESI/MS. T he generation of these impurities was attributed to the fluctuation of glucosyla tion in spiramycin biosynthesis. Nitrogen sources, ammonium in particular, were found to play an important role at the glucosylation. Aided with the information of LC-ESI/MS analysis and subsequent optimization of the culture medium, bette r culture medium of shake flask was designed, which leads to reduction of impuri ties by 22%～88%.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第2期275-278,共4页
Chinese Journal of Biotechnology
关键词
液质联用
螺旋霉素
杂质
优化
LC-ESI/MS, spiramycin, impurity, optimization
