摘要
为了探讨二氧化硫 (SO2 )吸入后是否可进入小鼠不同组织器官 ,运用高效液相色谱 (HPLC)荧光检测 (FD)法测定了SO2 动式吸入后雄性小鼠脑、心和肺组织中SO2 在体内的衍生物———亚硫酸盐含量 .组织匀浆液经还原、衍生和沉淀蛋白 ,取上清液进入色谱仪检测 .亚硫酸盐测定标准曲线在 0 12 6μg·mL- 1 ～ 12 6μg·mL- 1 有良好的线性关系 ,检测限为 0 0 4μg·mL- 1 (S N =3 ) ,测定方法的回收率在 97%～ 10 1%之间 ,日内和日间的精密度RSD低于 9% .分析结果表明 ,SO2 吸入后小鼠 3种器官组织中亚硫酸盐含量比对照组显著增加 (P <0 0 5 ) ,且与SO2 浓度呈明确的剂量效应关系 (r>0 92 ) .这说明SO2 被小鼠吸入后转化为亚硫酸盐并可分布到肺和其它器官如脑和心等 ,从而为SO2 是一种全身性毒物的观点提供支持 .此外 ,本文对HPLC荧光检测亚硫酸盐的方法作了改进 ,为研究SO2
The distribution of the sulfur dioxide (SO_2) permeated into the different organs in the mouse after SO_2 was inhaled was studied The concentration of the sulfite in brains, hearts and lungs of the male mice were measured by high performance liquid chromatographic (HPLC) with fluorescence detection (FD). Firstly, the protein was reduced, derived and deposited from the homogenates of brains, hearts and lungs tissue from mice. And then the mixture was centrifuged, and 5μL supernatant was directly injected into HPLC, where the flow phase was methanol-phosphoric acid(12∶88,V/V) ,and the exciting and emitting wavelength of fluorescence detection were 392nm and 478nm, respectively. The standard curve of the sulfite was greatly linear fit in the range from 0.126(μg·mL^(-1)) to 126(μg·mL^(-1)), and the minimal detectable concentration was 0.04μg·mL^(-1)(S/N=3) with the average recycle rates of method from 97% to 101%. The error-sensitivity within one day or between different days were less than 9%.The results showed that sulfite concentration in all the organs of mice in SO_2-exposed groups was significantly increased (P<0.05) compared with the controlled groups. And it was increased in a specific dose-dependent manner(r>0.92) which indicated SO_2 could transform into sulfite after it was inhaled, and distributed into lung and other organs such as brain and heart. These results supported the viewpoint that SO_2 was a systemic toxic agent. Moreover, the method of the measuration of sulfite by HPLC with fluorescence detection was improved which provided a more effective method to study the toxicological action of SO_2 and its derivatives.
出处
《环境科学学报》
CAS
CSCD
北大核心
2005年第3期346-350,共5页
Acta Scientiae Circumstantiae
基金
国家自然科学基金资助项目 (No .2 0 4770 2 3
No .3 0 2 3 0 3 10 )
山西省自然科学基金 (No .99112 3 )
