摘要
目的研究par-4反义寡核苷酸拮抗谷氨酸对PC12细胞中ERK1/2活性的下调作用及其抗凋亡作用。方法利用脂质体将par4反义寡核苷酸转染入PC12细胞。谷氨酸诱导PC12细胞凋亡。利用吖啶橙/溴化乙锭荧光染色观察PC12细胞形态,流式细胞分析评价凋亡百分率。Western印迹测定par4的蛋白质表达量和磷酸化的ERK1/2的蛋白表达量。结果(1)谷氨酸诱导PC12细胞中par-4蛋白表达上调,Par4反义寡核苷酸呈剂量依赖性地拮抗其上调(组间比较,P<0.01)。(2)谷氨酸诱导PC12细胞中磷酸化(Thr202/Tyr204)的ERK1/2蛋白水平下调,par-4反义寡核苷酸拮抗其上调(组间比较,P<0.01)。(3)Par-4反义寡核苷酸拮抗谷氨酸诱导的PC12细胞凋亡,如予ERK1/2阻断PD98059预处理,则其拮抗作用被下调(组间比较,P<0.05)。结论Par-4反义寡核苷酸拮抗谷氨酸诱导的PC12细胞凋亡,其机制可能与ERK的活化有关。
Objective To investigate the inhibition effects of par-4 antisense oligodeoxynucleotide on apoptosis of PC12 cell induced by glutamate and its signal transduction mechanism.Methods (1) Cationic lipid-mediated par-4 antisense oligodeoxynucleotide (Par-4-AS-ODN) was transfected into PC12 cells before they were treated with glutamate. Mismatch oligodeoxynucleotide (MS-ODN) were also transfected into cells as controls. (2) Morphological observation and the detection of anti-apoptosis effects of par-4-AS-ODN on PC12 cells were done with the Laser Scanning confocal Microscope by double staining the cells with acridine orange/ethidium bromide (AO/EB), addition to with flow cytometry. (3) Western blot was used to detect the protein levels of par-4 and phosphorylated ERK_ 1/2 at threonine-202 and Tyrosine-204. Results (1) Par-4-AS-ODN significantly suppressed up-regulation of the par-4 protein levels induced by glutamate in PC12 cells. (2) Par-4-AS-ODN could resist the decrease of phosphorylated ERK_ 1/2 (Thr202/Tyr204) induced by glutamate in PC12 cells. (3) Par-4 AS-ODN could inhibit apoptosis of PC12 cells induced by glutamate. But its inhibition effect could be eliminated by PD98059, a selective MEK_1 inhibitor which could inhibit phosphorylation of ERK_ 1/2. Conclusion Par-4 AS-ODN may inhibit apoptosis of PC12 cells induced by glutamate, and its inhibition effects may be medicated by the activation of ERK_1/2.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2005年第11期777-780,共4页
National Medical Journal of China
