摘要
目的:研究PD ECGF与宫颈鳞癌发生与发展的关系及作用机制。方法:采用原位杂交和免疫组化法,对87例宫颈鳞癌和2 3例正常及慢性宫颈炎组织PD ECGFmRNA、PD ECGF蛋白进行检测。结果:①PD ECGF在宫颈鳞癌细胞及间质细胞中均有表达,表达率分别为6 2 .0 7%、2 3.2 0 %。PD ECGFmRNA主要在宫颈鳞癌细胞中表达,表达率为5 7.4 7%。二者与宫颈鳞癌临床分期、组织分化程度明显相关(P <0 .0 5 )。②一致性检测发现宫颈鳞癌组织中PD ECGF基因的免疫组化与原位杂交检测结果关系密切(P <0 .0 1 )。结论:①PD ECGF由宫颈鳞癌细胞产生,在间质内发挥作用。②PD ECGF与宫颈鳞癌的发生、发展有密切关系,可作为判断宫颈鳞癌生物学行为和预后的重要指标。
Objective: To study the relationship between PD-ECGF expression and the pathogenesis and development of cervical squamous cell carcinoma. Methods:By using of in situ hybridization and immunohistochemistry, the PD-ECGF gene (PD-ECGFmRNA and PD-ECGF protein) were detected in 87 samples of cervical squamous cell carcinoma and 23 samples of normal cervical tissues and/or chronic cervicitis. Results:① The expression rate of PD-ECGF protein in squamous cell and stroma of cervical carcinoma was 62.07% and 23.20%, respectively. PD-ECGFmRNA mainly expressed in squamous cell and the expression rate was 57.47%. PD-ECGF protein and PD-ECGFmRNA were positively associated with tissue differentiation and clinical stage of cervical squamous cell carcinoma (P<0.05). ② The expression of PD-ECGF protein level was significantly correlated with that at mRNA level (P<0.01). Conclusions: ① PD-ECGF was produced in cervical squamous cell, but play a role in stroma cell. ② PD-ECGF is closely related to the pathogenesis and development of cervical squamous cell carcinoma, and can be a marker to ensure its biological behaviour and prognosis.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2005年第3期151-153,i001,共4页
Journal of Practical Obstetrics and Gynecology
基金
宁夏自然科学基金 (A40 1 9)
关键词
宫颈鳞癌
血小板源性内皮生长因子
原位杂交
免疫组化
Cervical squamous cell carcinoma
Platelet-derived endothelial cell growth factor
In situ hybridization
Immunohistochemistry
