摘要
报道了利用从生芽途径快速繁殖蝴蝶兰的研究结果,试验表明:培养基MS+BA1.0mg·L-1+NAA0.05mg·L-1+GA30.5mg·L-1能够诱导花梗休眠芽转化为营养芽.花梗芽在培养基MS+BA5.0mg·L-1+NAA0.05mg·L-1上能够诱导产生丛生芽.在丛生芽增殖阶段,以BA12.5mg·L-1+NAA0.05mg·L-1的增殖效果最好.BA、KT、ZT均能诱导产生"丛生芽",其中以ZT和BA的诱导效果最好.另外,在增殖阶段,外植体采用双芽接较单芽接的增殖效果好.在生根阶段以1 2MS+NAA0.5mg·L-1+w=0.2%的活性炭+φ=10%的椰子水的生根效果较佳,生根率可达100%,且植株生长健壮.试管苗移栽在水苔上的成活率较高,成活率达96 7%.
A study on in vitro rapid propagation through the clustered shoots of Phalaenopsis amabilis Bl.was reported in this paper.The results showed that in MS medium with BA 1.0 mg·L^(-1)+NAA 0.05 mg·L^(-1)+GA_3 0.5 mg·L^(-1),the dormant shoot of its flower stem could be induced to form vegetative shoot; and in MS medium with BA 5.0 mg·L^(-1)+NAA 0.05 mg·L^(-1),the clustered shoots could be induced from its flower stem shoot.During the proliferating period of clustered shoots,the medium with BA (12.5 mg·L^(-1)+)NAA 0.05 mg·L^(-1) showed the best effects.Furthermore,all the BA,KT and ZT could induce clustered shoots,but ZT and BA were the most efficient.Additionally,at the stage of proliferation,inoculation with double shoots has a better result than inoculation with single shoot.In rooting stage,the 1/2 MS with NAA 0.5 mg·L^(-1)+0.2% activated carbon(w/w)+10% coconut water (v/v)was efficient,with a rooting rate reaching 100% and strong plants.When transplanted on water moor,the tissue culture shoots had a higher survive rate reaching 96.7%.
出处
《海南大学学报（自然科学版）》
CAS
2005年第1期47-52,60,共7页
Natural Science Journal of Hainan University
