摘要
背景与目的 :研究木蹄层孔菌乙醇提取物(ethanolextractofFomesfomentarius,EEF)体内外对肿瘤细胞的抑制作用。 材料与方法 :噻唑蓝(3_[4,5_dimethylthiazol_2_yl]_2,5diphenyltetrazoliumbroide,MTT)法测定EEF对体外培养的HeLa细胞的生长的影响 ;流式细胞仪检测其体外对S180 细胞凋亡率的影响 ;建立腹水型S180肉瘤模型 ,测定EEF对荷瘤鼠生命延长率的影响 ;通过试剂盒检测EEF对正常小鼠几个重要器官丙二醛(Malondialdehyde,MDA)含量的影响。 结果 :EEF对体外生长的HeLa细胞有明显的抑制作用 ;能显著提高S180 细胞的凋亡率及荷瘤鼠的生存天数 ;能明显降低正常鼠肝脏、肾脏MDA含量。 结论 :EEF对肿瘤细胞有很强的抑制作用。
BACKGROUND&AIM: To study the inhibition of tumor cells by ethanol extract of Fomes fomentarius(EEF)both in vivo and in vitro. MATERIAL AND METHODS: The influence of EEF on HeLa cells was assayed by MTT method.The effect of EEF on the apoptosis of S 180 cells was analysed by FCM.The effect of EEF on the extending rates of mice carrying S 180 tumor was investigated.MDA contents of several principle issues were observed,after the mice were dealed with EEF.RESULTS: EEF had obvious inhibitory activity to HeLa cells in vitro in a dose-dependent manner;could remarkably increase the apoptosis of S 180 cells,the extending rates of mice carrying S 180 tumor;MDA contents of liver,kindey were increased significantly,after the normal mice were treated by EEF. CONCLUSION: Ethanol extract of Fomes fomentarius has obvious inhibition on tumor cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2005年第2期104-106,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
江苏省药物植物生物技术重点实验室重点项目(No.ZXL0512)
重要生物资源保护与利用研究安徽省重点实验室专项基金
关键词
木蹄层孔菌
抗肿瘤
抑制作用
Fomes fomentarius
anti-tumor
inhibitory effect
