摘要
背景与目的 :探讨宫颈癌前病变和宫颈癌的发生发展与人乳头状瘤病毒及单纯疱疹病毒(HerpesSimplexVirus,HSV)、巨细胞病毒(Cytomegalovirus,CMV)的关系。 材料与方法 :对81例不同宫颈病变组织进行HPV16/18和HPV6/11原位杂交 ,同时对103例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。 结果: 病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16/18与不同宫颈病变组织原位杂交阳性率平均为51.1 % ,HPV6/11的则为64.7 %。经PCR检测 ,HPV16/18、HPV6/11、HSV、CMV在不同宫颈病变组织中的阳性率分别为21 %、4 %、23 %、0 %。 结论 :HPV感染具有特定的组织学部位 ,HSV可协同HPV16/18恶性转化宫颈上皮细胞。
BACKGROUND&AIM: The carcinogenesis of the human cervical precancerous lesion,cervical carcinoma is known closely associated with human papillomavirus(HPV).The purpose of this study was is to identify whether Herpes Simplex Virus(HSV)and Cytomegalovirus(CMV)play a co-factor role in the carcinogenesis. MATERIAL AND METHODS: Eighty-one cases of various cervical lesions were analyzed by HPV6/11,HPV16/18in situ hybridization.Meanwhile,HPV,HSV and CMV were determined in103cases of various cervical lesions. RESULTS: The results show that the distribution of positive hybridization signal was consistent with the distribution of koilocytes in HE stain.Of these cervical specimens investigated,the positive rates of HPV16/18and HPV6/11using ISH were51.1%and64.7%,respectively,the infection rates of HPV16/18,HPV6/11,HSV and CMV using PCR were21%,4%,23%and0%,respectively. CONCLUSION: The co-operation effect of HPV and HSV might occur in the oncogenesis of human cervical carcinoma.
出处
《癌变．畸变．突变》
CAS
CSCD
2005年第2期107-109,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
宫颈癌
人乳头瘤病毒
原位杂交
cervical carcinoma
human papillomavirus
in situ hybridization
