摘要
目的 探讨肿瘤抑制基因杂合性缺失(LOH)在肝原发性淋巴瘤(PHL)发生中的作用。方法 采用显微组织切割术 从石蜡组织切片中提取DNA,在PCR基础上进行毛细管电泳DNA测序,对9例手术切除的PHL标本进行了视网膜母细胞瘤 1基因(Rb1)、腺瘤性息肉病基因(APC)、结直肠癌突变基因(MCC)、结直肠癌缺失基因(DCC)等4种肿瘤抑制基因(TSGs) LOH检测。结果 B细胞系PHL中APC基因LOH发生率为50.0%,其余3种基因未检测到LOH,T细胞系PHL中LOH发生 率为APC(33.3%)、Rb1(50.0%)、DCC(20.0%)、MCC(0.0)。结论 PHL中有较高频的APC和Rb1基因LOH,提示在PHL 的形成过程中APC和Rb1基因的变异起重要作用。
Purpose To investigate the possible role of loss of heterozygosity (LOH) of tumor suppressor genes in the development of primary hepatic lymphoma (PHL). Methods Nine cases of PHL were analyzed for LOH in four tumor suppressor genes,including APC,MCC,DCC and Rb1,by means of microdissection-based polymerase chain reaction (PCR) and DNA sequencing using capillary electrophoresis. Results In B cell PHL,the frequency of LOH was 50% at APC,no LOH was found in the other three TSGs,but in T cell PHL,the frequency of LOH was 33.3% at APC,50.0% at Rb1,20.0% at DCC,no LOH was found at MCC. Conclusions LOH is a common event in PHL,which indicted that LOH of tumor suppression genes(APC and Rb1 genes) may play a important role in origination and progress of PHL.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2005年第1期47-49,共3页
Chinese Journal of Clinical and Experimental Pathology
