摘要
目的 研究树突状细胞在MODS免疫器官损伤中的病变特点。方法 运用光镜、电镜与免疫组化(CD1a、CD205及 MIDC -8标记)方法,观察小鼠MODS模型中胸腺树突状细胞的变化特点。结果 致伤早期胸腺树突状细胞大量增生、机能活 跃伴淋巴细胞大量凋亡;在缓解期(3～7天)胸腺及树突状细胞恢复正常;MODS期(8～12天)树突状细胞数量再次增加,但 功能减弱。同时伴有较多树突状细胞和淋巴细胞变性凋亡。结论 树突状细胞病变是MODS免疫系统损伤的首发病变,树 突状细胞的数量与机能变化与MODS模型病程具有高度平行,提示树突状细胞病变与MODS启动机制和病程转归有重要关 系。
Purpose To study the pathological features of the mouse thymic dendritic cells (DCs) in multiple organ dysfunction syndrome (MODS). Methods The changes in mouse thymic DCs in MODS were studied by light microscopy,electronic microscopy and immunohistochemical stains (CD1a,CD205 and MIDC-8). Results In the early stage,the hyperplasia and the active function of DCs in the thymus were found together with the apoptosis in a large number of lymphocytes. In the day 3 to 7,the content of DCs returned to normal level. In the day 8 to 12 (MODS stage),the content of DCs increased but the function decreased. Conclusions The pathologic change in DCs might be the earliest changes of immune organs in MODS,and the changes were consistented with the course of mouse MODS. Thymic dendritic cells may play an important role in the initiation and development of MODS.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2005年第1期95-99,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
全军"十五"科研基金资助项目(01MA210)
