摘要
采用Tris-PEG法、电击转化法和TSS方法对5株野生短芽孢杆菌进行了转化.其中,Tris-PEG法转化短芽孢杆菌50和735,电击转化短芽孢杆菌50取得成功,转化率为102个转化子/μgDNA.
Tris-polyethylene glycol(PEG) method,electroporation,and TTS were carried out for introducing plasmid DNA into 5 strains of wild Bacillus brevis.pBKE50 could be transformed into Bacillus brevis 50 and 735 by Tris-polyethylene glycol (PEG) method,and incorporated into Bacillus brevis 50 by electroporation.a transformation frequency is 102 transformants/μg DNA.
出处
《吉首大学学报（自然科学版）》
CAS
2004年第4期35-38,共4页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省教育厅青年基金资助项目(03B031)
