摘要
目的 检测上海地区幽门螺杆菌 (Hp)感染患者中Hp菌株iceA、babA2的分布特征 ,探讨与Hp临床感染结局相关的菌株基因型。 方法 1 4 1株Hp菌株分离自 4 3例慢性胃炎 (CG)、4 7例十二指肠球部溃疡 (DU)、30例胃溃疡 (GU)和 2 1例非贲门部胃癌患者的胃镜活检标本。采用PCR方法检测Hp菌株的iceA、babA2、cagA和vacA基因型。 结果 1 4 1株Hp菌株中 ,iceA1、iceA2和babA2的总检出率分别为 74 .5 % (1 0 5 /1 4 1 )、1 5 .6 % (2 2 /1 4 1 )和 6 3.8% (90 /1 4 1 ) ,其中 2例 (1 .4 % )为iceA1、iceA2均阳性 ,1 6例 (1 1 .3% )为iceA1、iceA2均阴性。DU组的babA2检出率显著高于GU组 (74 .5 %比5 0 .0 % ,P =0 .0 2 8) ,DU组的cagA+ /babA2 + 检出率亦显著高于GU组 (70 .2 %比 4 6 .7% ,P =0 .0 39)。其余疾病组之间的babA2检出率差异无显著性。未发现不同临床疾病与iceA基因型的相关性。结论 上海地区Hp感染者的菌株基因型主要是iceA1 + /babA2 + ,babA2在DU和GU的发病机制中起不同作用。
Objective To determine the distribution of Helicobacter pylori (H.pylori) iceA, babA2 in patients in Shanghai and explore the association of H. pylori strain genotype with its clinical outcome after infection. Methods A total of 141 H. pylori strains was isolated from gastric biopsy samples of 43 patients with chronic gastritis, 47 patients with duodenal ulcer (DU), 30 patients with gastric ulcer(GU) and 21 patients with non cardia gastric carcinoma. The iceA, vacA, cagA, and babA2 genotypes were determined by polymerase chain reaction (PCR). Results iceA1, iceA2 and babA2 were detected in 74.5% (105/141) , 15.6% (22/141) and 63.8% (90/141) of the 141 H.pylori strains, respectively, while 2 of isolated H. pylori strain (1.4%) were positive for both iceA alleles and 16(11.3%) were negative for both iceA alleles. The prevalence of babA2 and the combined genotype of babA2 and cagA in H. pylori isolated from DU patients were significantly higher than that in GU patients (74.5% vs. 50.0% for babA2, P =0.028; 70.2% vs. 46.7% for babA2 and cagA, P =0.039). There was no significant difference in prevalence of babA2 among other disease groups. No association of different clinical diseases with iceA genotype was detected. Conclusions The most common genotype of H.pylori strains isolated from patients in Shanghai is iceA1 +/babA2 +. babA2 may play different role in the pathogenesis of duodenal ulcer and gastric ulcer. No association between iceA status and clinical outcome of H.pylori infection was confirmed in our study.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2004年第6期322-325,共4页
Chinese Journal of Digestion
