摘要
结果表明，粉防己碱（Ｔｅｔ）非常明显地阻断Ｋ＋去极化导致的胎鼠大脑［Ｃａ２＋］ｉ含量的增加。经不同浓度Ｔｅｔ处理的大脑细胞加入ＫＣｌ后，［Ｃａ２＋］ｉ含量仍基本维持在静息状态下水平。对［Ｃａ２＋］ｉ的阻断程度与Ｔｅｔ浓度无关。Ｔｅｔ对Ｌ－谷氨酸（Ｌ－Ｇｌｕ）所致大脑［Ｃａ２＋］ｉ升高亦有明显抑制作用。１０－７ｍｏｌ·Ｌ－１浓度的Ｔｅｔ还降低ＢａｙＫ８６４４引起的［Ｃａ２＋］ｉ上升高。Ｔｅｔ能抑制高Ｋ＋，二氢吡啶类钙激动剂及兴奋性神经递质导致的胎鼠大脑［Ｃａ２＋］ｉ含量增加，提示Ｔｅｔ对神经细胞损伤可能有一定保护作用。
Cytosolic free Ca2+─[Ca2+]i
was measured in dissociated cerebral cells isolatedfrom fetal rats
using the fluorescent indicater Fura-2.Increases in[Ca2+]i occurred
rapidly followingexposure of the cells to 50 mmol·L-1 KCl,10-7
mol·L-1 Bay K 8644 and 200 umol·L-1 glu-tamate(Glu ).[ Ca2+]i
elevation produced by K+ -depolarization was blocked completely by
pretreat-ment with tetrandrine(Tet)of various concentrations(10-9～
10-7 mol·L-1,final).Tet showedsignificant inhibitory effect
on[Ca2+]i increases stimufated by Glu in
concentration-dependentmanner.With Tet 10-7 mol·L-1,Bay K
8644-induced increases in[Ca2+]i were reduced_markedly.These results
indicate that Tet can block the increases in [Ca2+]i in fetal rat
cerebral cellsinduced by Ca2+ agonists,suggesting that this drug may
have a protective effect on cerebral cellinJury.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第7期492-496,共5页
Acta Pharmaceutica Sinica
