摘要
目的 了解术前化疗对胃腺癌细胞凋亡的影响。方法 采用抽签法术前随机分为口服脱氧氟尿苷(5′- DFUR)组(18例)、静脉输注5- FU +CF组(16例)及对照组(2 0例)。化疗结束后第1～2天手术。采用免疫组织化学及TUNEL法检测癌细胞增殖指数(PI)及凋亡细胞指数(AI)。结果 PI在5′-DFUR组(40±13)、5 - FU +CF组(41±15 )明显低于对照组(5 8±16 ) ,差异有统计学意义(P =0 . 0 0 0 ) ;AI在5′- DFUR组(14±9)、5 - FU +CF组(14±10 )明显高于对照组(7±7) ,差异有统计学意义(P =0 . 0 17)。4 4例获得随访。总的0 . 5、1、2年生存率分别为93%、86 %、70 % ,0. 5、1、2年无瘤生存率分别为89%、77%、6 7%。3组术后生存时间差异无统计学意义。结论 术前3～5d短程口服5′- DFUR化疗可诱导胃腺癌细胞凋亡,降低癌细胞增殖指数,但不能改善术后生存情况。
Objective To study apoptosis and proliferation of gastric adenocarcinoma cells induced by 5′-deoxy-5-fluorouridine (5′-DFUR).Methods Patients of gastric carcinoma were divided randomly into three groups before operation: group 1 (18 cases) receiving 5′-DFUR 800～1200 mg/d×3-5 d po, group 2 ( 16 cases ) treated by 5-FU 500 mg+CF 200 mg/d×3-5 d and group 3 (20 cases) serving as control.One or two days after chemotherapy, patients were operated on. The proliferation index (PI) and apoptosis index (AI) of gastric carcinoma cells were evaluated by in-situ TUNEL and En Vision PCNA immunohistochemical method.Results PI in group 1 (40±13) and group 2 (41±15) were lower than that in control (58±16), ( P =0.000). AI in group 1 (14±9) and group 2 (14±10) were significantly enhanced than that in group 3 (7±7), ( P =0.017).A total of 44 patients were followed up, with 0.5-? 1-? 2-year survival rates of 93%? 86% and 70%, respectively.The disease free 0.5-? 1-? 2- year survival rates were 89%? 77% and 67%, respectively.The survival rates among three groups were not statistically different.Conclusion Preoperative short course 5′-DFUR administration do not improve prognosis of postoperative patients with gastric cancer.
出处
《中华普通外科杂志》
CSCD
北大核心
2005年第3期184-186,共3页
Chinese Journal of General Surgery
基金
浙江省教育厅资助项目 (2 0 0 10 5 3 6)
