摘要
目的:明确三种中药有效成分三七皂甙(PNS)、黄芪皂甙(As)及黄芩甙(Ba)单用或配伍应用对永生化的人胃黏膜上皮细胞GES-1以及经过甲基硝基亚硝基胍(MNNG) 转化后的GES-1细胞(MC细胞)增生能力的影响. 方法:以MNNG转化GES-1细胞,转化后细胞简称MC 细胞.分别以不同浓度的三七皂甙、黄芪皂甙及黄芩甙处理GES-1细胞及MC细胞,四甲基谷氮唑盐(MTT)法及软琼脂集落形成试验检测三种药物单用及配伍对细胞增生活性的影响.Annexin V及PI双染,流式细胞仪检测三药单用及配伍对MC细胞凋亡/坏死的影响;电镜和Hoechst33258及PI双染荧光显微镜观察凋亡细胞形态. 结果:PNS、AS及Ba对GES-1和MC细胞的增生活性有明显的抑制作用,并有随剂量增加作用增强的趋势.三药配伍应用对2种细胞的抑制作用增强(P<0.05或P<0.01vs三药单用组),还能明显抑制MC细胞的软琼脂集落形成能力(P<0.05或P<0.01 vs MC细胞对照组). 三药单用可引起MC细胞凋亡及死亡比例增加并呈一定的时间依赖性,配伍应用作用强于单独应用(P<0.05 或P<0.01 vs 三药单用组),增强程度从大至小依次为PNS+AS+Ba>PNS+AS>AS+Ba>PNS+Ba. 结论:三种中药有效成分单用及配伍可显著抑制MC细胞的增生并引起细胞死亡;引起细胞死亡的机制部分是通过诱导凋亡实现的;不同药物配伍应用的增效程度不同.
AIM: To investigate the cytotoxic effects of Panax Notogengsing Saponin (PNS), Astragalin Saponin (AS) and Baicalin (Ba), the main active components from 3 Chinese herbal medicines, on an immortalized human gastric epithelial cell line GES-1 and the N-methyl-N'-nitro-N- nitrosoguanidine (MNNG) transformed GES-1 cells (MC cells). METHODS: GES-1 cells were transformed by 2×10-5mol/L MNNG. The transformed cells were named as MC cells. MC cells were treated with the 3 medicines alone or in different combinations. Cell viability was detected by MTT assay and soft agar colony formation assay, while apoptosis/ necrosis were determined by electron microscopy, fluoros-copy and flow cytometry with annexin V staining. RESULTS: PNS, AS and Ba inhibited the growth of GES-1 and MC cells in a dose and time-dependent manner. The colony formation efficiency in soft agar of MC cells was also decreased after treated with PNS, AS, Ba alone or in combination (P<0.05 or P<0.01 vs control group). The 3 medicines induced apoptosis and/or necrosis of MC cells time-dependently. These effects were greatly enhanced when two or three of these medicines were used together (P<0.05 or P<0.01 vs 3 medicines used alone). The most potent combination was PNS+AS+Ba, followed by PNS+AS, AS+Ba and PNS+Ba, in a decreasing order. CONCLUSION: PNS, AS and Ba can inhibit cell growth and induce cell death on MC cells. The cell death is partially caused by cell apoptosis. These effects can be enhanced to different extent by different combinations of these drugs.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第4期477-482,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助课题资助项目
No.30100249~~
