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胃腺癌细胞株SGC7901中Fas及FasL的基因转录与表达

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摘要 目的:探讨胃腺癌细胞株SGC7901中Fas及FasL基因转录与表达情况,为胃癌与Fas及FasL基因的相关研究提供帮助方法:利用反转录聚合酶链反应(RT—PCR)检测胃腺癌细胞株SGC7901中Fas及FasL基因mRNA转录情况.采甩免疫细胞化学染色鉴定胃腺癌细胞株SGC7901中Fas及FasL基因有无蛋白表达及其表达程度. 结果:从提取的胃癌细胞总RNA中分别扩增出Fas及FasL 的DNA片段,免疫细胞化学染色亦显示胃腺癌细胞株SGC7901中存在Fas及FasL蛋白表达产物. 结论:中国人胃腺癌细胞株SGC7901中存在Fas及FasL基因的mRNA转录及蛋白的表达.
出处 《世界华人消化杂志》 CAS 北大核心 2005年第4期565-566,共2页 World Chinese Journal of Digestology
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