摘要
目的:从人肝细胞癌中分离肝癌细胞并对其体外诱导分化特性进行分析,试图得到有关导致肝癌发生的“癌干细胞”的相关资料。方法:首先将人原发性肝癌组织小块在裸鼠皮下过继接种,然后将生成的肿瘤进行原代培养并得到单层生长的肿瘤细胞系,利用体外诱导实验对其形态及分子表型的变化情况进行观察和分析。结果:所分离获得的细胞系具有肝肿瘤细胞的特征,但也表达某些干细胞的分子标志如c met。体外诱导分化实验提示胰岛素/氢化可的松、二甲亚砜可能具有诱导该细胞向成熟肝细胞方向分化的作用,诱导后的细胞又重新表达葡萄糖6 磷酸酶,白蛋白表达明显升高。结论:人P2 HCC细胞具有强大的增殖能力,体外实验提示其具有一定的向成熟肝细胞分化的潜能。这些细胞的发生与肝癌干细胞及肝细胞的关系尚待进一步研究。
Objective:To isolate hepatoma cells from human primary hepatocacinoma and characterize their inductive differentiation in vitro, searching for data about 'hepatic cancer stem cell' which results in hepatocarcinogenesis. Methods: Fresh surgical human primary hepatocarcinoma specimen was implanted i.p. in NOD/SCID mouse and was allowed to grow. Then the neoplasm was harvested and cells were isolated and a cell line named P2-HCC was obtained. P2-HCC was induced with medium including insulin/hydrocartisone or DMSO, and then the differentiation of phenotype and changes of some proteins expression, such as albumin, c-met, G-6-P of P2-HCC were observed. Results: P2-HCC cell line was a hepatocarcinoma cell line which displayed characteristics of cancer cell. P2-HCC expressed c-met, which was regarded as a signature of liver stem cell or liver progenitor cell. When insulin/hydrocartisone and DMSO existed in medium, cells could differentiate into mature hepatocyte and lead to the reexpression of G-6-P, distinctly raising expression of albumin. Conclusion: The results suggest that P2-HCC can differentiate into mature hepatocyte, but the correlation between P2-HCC and liver cancer stem cell remain to be probed further.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第3期247-250,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金( 30470876
30270668
30270603
30200138)
上海市科委重大项目(03DJ14020)
关键词
癌
肝细胞
肿瘤细胞
培养的
细胞分化
carcinoma,hepatocarcellular
tumor cells,cultured
cell differentiation
