期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Separase上调对肿瘤细胞染色体分离作用的研究及条件表达载体的构建 被引量：4

Effects of up-regulation of separase/esp1 on tumor chromatid separation and construction of a conditional expression vector
下载PDF
导出
摘要 目的　研究esp1在肺腺癌细胞株spc细胞中对染色体分离的作用,为肿瘤的基因治疗探索新的方法。方法 　将已有的含esp1基因的EGFP N1质粒转染spc细胞,检测其转染效率及对染色体分裂相的影响。同时构建能依赖四环 素调控的含有esp1基因的pTRE- d2EGFP质粒载体。结果　表达载体EGFP N1 esp1转染成功,染色体异常分裂相减少。表 达载体pTRE -d2EGFP esp1构建成功。结论　esp1的上调表达对于肿瘤的异常染色体分裂相有一定的抑制作用,对于肿瘤 治疗有潜在的应用价值。 Objective To construct an expression vector containing esp1 in order to investigate the effects on the chromatid separation. Methods A conditional plasmid vector containing esp1 was constructed, and was transfected into spc cells. The expression level of esp1 was detected by fluorescence microscopy, and the chromosomes were observed. Results EGFP-N1-esp1 was expressed in spc cells, and improper chromosome segregation was reduced. The plasmid of pTRE-d2EGFP-esp1 was constructed successfully. Conclusion Up-regulation of esp1 has inhibitory effects on the abnormal chromatid separation of tumors and may be potential in the treatment of cancer.
作者 由莉越 刘昕
机构地区 第三军医大学基础医学部分子遗传学教研室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2005年第6期497-499,共3页 Journal of Third Military Medical University
关键词 spe细胞 esp1 转染 四环素 spc cell esp1 transfection tetracycline
分类号 R394.33 [医药卫生—医学遗传学] R394.2 [医药卫生—医学遗传学]
  • 相关文献

参考文献9

  • 1萨姆布鲁克J 弗里奇EF 曼尼阿蒂斯T.分子克隆实验指南[M].北京：科学出版社,1999..
  • 2Ciosk R, Zachariae W, Michaelis C, et al. An ESp1/PDs1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast[J]. Cell, 1998, 93(6): 1067-1076.
  • 3Jensen S, Segal M, Clarke D J, et al. A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1[J]. J Cell Biol, 2001, 152(1): 27-40.
  • 4Uhlmann F, Wernic M, Poupart E, et al. Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast[J]. Cell, 2000, 103(3): 375-386.
  • 5Waizenegger I, Gimenez Abian J F, Wernic D, et al. Regulation of Human Separase by Securin Binding and Autocleavage[J]. Curr Biol, 2002, 12(16): 1368-1378.
  • 6Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters[J]. Proc Natl Acad Sci U S A, 1992, 89(12): 5547-5551.
  • 7Gossen M, Freundlieb S, Bender G, et al. Transcriptional activation by tetracyclines in mammalian cells[J]. Science, 1995, 268(5218): 1766-1769.
  • 8Gupta S, Schoer R A, Egan J E, et al. Inducible,reversible, and stable RNA interference in mammalian cells[J]. Proc Natl Acad Sci U S A, 2004, 101(7): 1927-1932.
  • 9Wiznerowicz M, Trono D. Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference[J]. J Virol, 2003, 77(16): 8957-8961.

共引文献42

同被引文献36

  • 1罗勇军,刘昕.实时荧光定量PCR标准品的制备及应用[J].重庆医学,2005,34(3):414-415. 被引量：37
  • 2CHESTUKHIN A,PFEFFER C,MILLIGAN S,et al.Processing,localization,and requirement of human separase for normal anaphase progression[J].Proc Natl Acad Sci USA,2003,100(8):4574-4579.
  • 3NASMYTH K,PETER J M,UHLMANN F.Splitting the chromosome:cutting the ties that bind sister chromatids[J].Scince,2000,288(5470):1379-1385.
  • 4UHLMANN F.Secured cutting:Controlling separase at the metaphase to anaphase transition[J].EMBO Rep,2001,21(6):487-492.
  • 5HENGARTNER M O.The biochemistry of apoptosis[J].Nature,2000,407(6805):770-776.
  • 6UHLMANN F,WERNIC D,POUPART M A,et al.Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast[J].Cell,2000,103(3):375-386.
  • 7CHEN F,KAMRADT M,MULCAHY M,et al.Caspase proteolysis of the cohesion component RAD21 promotes apoptosis[J].J Biol Chem,2002,277(19):16775-16781.
  • 8PATI D,ZHANG N,PLON S E.Linking sister chromatid cohesion and apoptosis:role of RAD2[J].Mol Cell Biol,2002,22(23):8267-8277.
  • 9ATIENZA J M,ROTH R B,ROSETTE C,et al.Suppression of RAD21 gene expression decreases cell growth and enhances cytotoxicity of etoposide and bleomycin in human breast cancer cells[J].Mol Cancer Ther,2005,4(3):361-368.
  • 10CIOSK R,ZACHARIAE W,MICHAELIS C,et al.An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast[J].Cell,1998,93(3):1067-1076.

引证文献4

二级引证文献1

第三军医大学学报
2005年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部