摘要
目的:研究不同反应体系对NDPK酶活性测定结果的影响。方法:分别以(UTP+ADP)和(ATP+UDP)作为反应底物,加入自制的rhNDPK-A蛋白,以100mmol/L磷酸二氢钾、20mmol/L乙酸、5mmol/L四丁基溴化铵,pH 5 0作为流动相A液, A液+25%乙腈作为B液,等梯度混合,C18柱,柱温25℃,流速1 0ml/min,检测波长254nm。结果:以(UTP+ADP)和(ATP+UDP)作为反应底物测定的酶比活分别为210U/mg、860U/mg。结论:不同反应体系对HPLC法测定NDPK酶活性有显著差异。
Objective:To study the activity differences of NDPK in two reactive systems.Methods:HPLC-UV determination was employed with C_(18) column at 25℃. The mobile phase A was 100 mmol/L KH_2PO_4, 20 mmol/L actetic acid, 5 mmol/L tetrabutylamino bromide, pH 5.0. The mobile phase B was 25% acetonitrile dissolved in mobile phase A. The wave length for determination was 254 nm.Results:The specificactivity of rhNDPK-a in (UTP+ADP) and (ATP+UDP) systems was 210 U/mg and 860 U/mg respectively.Conclusion:There was striking difference between (UTP+ADP) and (ATP+UDP) systems in measuring NDPK activity by HPLC.
出处
《中国卫生检验杂志》
CAS
2005年第4期390-391,共2页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金小额探索项目 (No 30371661)
国家自然科学基金面上项目(No 30400071)
