摘要
研究益气化瘀方及其拆方药理血清对诱导凋亡椎间盘纤维环细胞凋亡率的调控情况。采用TUNNEL法和流式细胞仪技术检测不同药物作用下纤维环细胞凋亡率的变化,比较分析益气化瘀方及其拆方益气方、化瘀方药理血清,以及IGF- Ⅰ对抗-Fas抗体诱导凋亡大鼠椎间盘纤维环细胞凋亡率的影响。结果显示,益气化瘀方可降低细胞凋亡指数,作用低于IGF -Ⅰ组,有显著性差异(P<0. 05)。提示益气化瘀方及其拆方可以调控细胞凋亡率,调控细胞的凋亡可能是其治疗颈椎病的机理之一。
To study the effects of the medicated serum of 'Yiqi Huayu Decoction' and its parting formulae on intervertebral disc cell apoptosis, TUNNEL method and flow cytometer technique were adopted to detect the apoptic rate and the effects of IGF-Ⅰon the intervetebral disc cell apoptosis due to Fas antibody. Results: The Decoction could apoptosis index, with better effects than IGF-Ⅰ(P<0.05). It is revealed that this Decoction and its parting formulae can reduce the apoptosis rate to treat cervical spondylosis.
出处
《上海中医药杂志》
北大核心
2005年第4期50-52,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目 ( 30171170 )
上海市"启明星"基金资助项目(01QB140390)
关键词
益气化瘀方
椎间盘
颈椎病
纤维环细胞
凋亡率
Yiqi Huayu Decoction'
intervertebral disc
cervical spondylosis
fibrotic cell
apoptosis rate
