摘要
采取高效液相色谱(HPLC)与质谱(MS)连用技术研究呋喃唑酮[3 (5 硝基糠醛缩氨基) 2 唑烷酮]及其主要代谢产物3 氨基 2 唑酮(AOZ)在罗非鱼体内的残留规律。该方法对呋喃唑酮及其代谢物AOZ的检出限分别为10μg/kg、1μg/kg。给罗非鱼投喂剂量为30mg/(kg·d)的呋喃唑酮药饵7d.结果表明,罗非鱼肌肉中呋喃唑酮和AOZ的含量分别在停药6h后和停药"零时"达到最高,分别为413.00±91.68μg/kg、31.15±9.68μg/kg,24h后呋喃唑酮含量就低于检出限,而肌肉中AOZ的含量在528h后才低于1μg/kg。鱼肌肉中呋喃唑酮和AOZ的消除半衰期分别为9.34h、38.2h,平均消除速率分别为22.7μg/(kg·h)、0.058μg/(kg·h)。由实验结果可以看出,鱼肌肉中呋喃唑酮代谢很快,而AOZ却很难消除。考虑到呋喃唑酮的代谢物AOZ在罗非鱼体内不容易消除,在本实验条件下,建议给罗非鱼投喂呋喃唑酮药饵的停药期至少在22d。
The residues of furazolidone [3-(5-nitrofurfurylidenamino)-2-oxazolidinone] and its main metabolite 3-amina-2-oxazolldinone (AOZ) in tilapia were first studied by HPLC/MS . The detection limit of furazolidone and AOZ were 10 μg/kg,1 μg/kg respectively. After oral dose of 30 mg/(kg·d) for 7 days,the maximum level of furazolidone in tilapias was 413.00±91.68 μg/kg after 6 h,but that of AOZ reached maximum (31.15±9.68 μg/kg) when stopping giving drug;the concentration of furazolidone was lower than 10 μg/kg after 24 h,but that of AOZ was just lower than 1 μg/kg after 528 h; the elimination half-life of furazolidone and AOZ were 9.34 h and 38.2 h respectively,and the rate of speed were 22.7 μg/(kg·h) and 0.058 μg/(kg·h) respectively. The results show that AOZ is very hard to eliminate compared to furazolidone. In view of AOZ is very hard to eliminate, the predicted withdrawal time of furazolidone for tilapia was 22 d at least in this condition.
出处
《上海水产大学学报》
CSCD
北大核心
2005年第1期35-39,共5页
Journal of Shanghai Fisheries University
基金
973课题(2002CB412402)
中国科学院重大项目(KJCX315W-215)
