摘要
目的:建立奥沙利铂反相高效液相色谱(RP-HPLC)含量测定方法。方法:采用RP-HPLC测定奥沙利铂的含量,色谱柱:kromasil C18柱(150mm×4.6mm),检测波长250nm。流动相:甲醇-水(5:95),流速:1.0ml/min。结果:本法线性范围为20μg/ml-100μg/ml,r=0.9999。日内和日间精密度RSD分别为0.71％和0.97％(n=5)。平均回收率为99.82％,RSD为0.89％。结论:该测定方法专属性强,操作简便,准确度高,精密度高,可作为奥沙利铂的含量测定方法,以控制其原料及制剂的质量。
Objective: To establish a RP-HPLC method for determination of Oxaliplatin content. Methods: The content of Oxaliplatin was determined by RP-HPLC. kromasil C18 column was used. The detection wavelength was set at 250nm. The mobile phase consisted of methanol-water(5:95) with the flow rate 1.0ml/min. Results: The method was linear within the range of 20-100μg/mL, r=0.9999. The within- day and between-day precisions were 0.71% ( n=5 ) and 0.97% ( n=5 ) respectively. The average recovery of the assay was 99.82% and RSD was 0.89%. Conclusion: The method appeared to be simple and rapid, highly accurate and precise. It can be used for quantitative determination of Oxaliplatin and its preparation.
出处
《抗感染药学》
2005年第1期24-24,26,共2页
Anti-infection Pharmacy
