摘要
用浓度（ｍｍｏｌ／Ｌ）１０、２５、５０、１００、１５０的ＡＯＴ（气溶胶ＯＴ）组成的反胶团对猪心肉糜提取液中的细胞色素Ｃ进行了萃取。结果为１０ｍｍｏｌ／Ｌ组几乎没有萃取效果，在２５～１５０ｍｍｏｌ／Ｌ浓度下的反胶团均有明显的萃取效果，萃取率（反萃取水相中的含量与原液中的含量的比值）还原型细胞色素Ｃ为２５％～３２％，氧化型细胞色素Ｃ为２８％～４２％．
In this article,the extraction of cytC from the prok heart extract was extracted byusing different AOT concentrations(10,25,50,100,150 mmol/L).It shows that when AOT con-centration is 10 mmol/L,the afffect of extraction is almost zero,and when AOT concentration is 25～150 mmmol/L,the affect is distinct.The extract ratio(expressed in the proportion of the cytCamount in the back extract water phase to that of the first extract)of reductive type cytC is 25%～32%,that of oxidative type cytC is 28%～42%.
关键词
猪心
气溶胶
反胶团
萃取
细胞色素C
pork heart extract
AOT
reverse micelle
extraction
cytochrome C
