摘要
在MS+蔗糖60g/kg的固体培养基中加入不同浓度的多效唑(PP333),对组培唐菖蒲球茎形成有不同程度的促进作用,其中浓度为30mg/kg的球茎形成较早;浓度为3和10mg/kg的形成球茎较大。在多效唑10mg/kg的培养基上,接种芽的长度不同对球茎形成也有影响,3~5cm的芽球茎形成较早;太长的芽,球茎形成反而推迟。活性炭对多效唑有明显吸附作用,不仅球茎形成推迟,球茎鲜重也下降。
Paclobutrazol at different concentrations improved gladiolus corm formation obviously when the shoots were cultured on the solid agar mediums which did not contain any other regulators (MS+sucrose 60g/kg). Corms formed earlier on the medium which contained 30mg/kg paclobutrazol, and corms formed larger when paclobutrazol was at 3~10mg/kg. Different length of shoots had some effect on corm formation when the shoots were cultured on the same medium which contained 10 mg/kg paclobutrazol. The inoculated shoots at 3~5cm formed earlier and larger, too long shoots delayed corm formation relatively. Active carbon had absorption effect on paclobutrazol obviously. It delayed corm formation and reduced fresh weight of corm.
出处
《园艺学报》
CAS
CSCD
北大核心
1994年第3期288-292,共5页
Acta Horticulturae Sinica
关键词
唐菖蒲
组织培养
多效唑
球茎形成
Gladiolus
Tissue culture
Paclobutrazol
Corm formation