摘要
In order to improve the diagnostic efficiency of the hepatitis C in China, the DNA fragments of core, C33c,NS4 in Hepatitis C virus(HCV) were connected and subcloned to pET24a(+). The pET24a-CCN was highly expressed with the inducement IPTG in the E.coli BL21(DE3). In 1L bacterial culture medium,30mg purified recombinant proteins were available using His Binding Resin under denatured condition. Used as diagnostic antigen to detect the human hepatitis C by ELISA, The results showed that the 100% positive coincidence rate (66/66) and 94.5% negative coincidence rate (86/91) were obtained. To detect 46 positive HAV sera and 46 positive HBs sera, all the results were negative. At the same time, the freeze-dried recombinant antigen detects 66 HCV positive sera, all the results were positive. Therefore, the recombinant antigen was sensitivity, specialty and stability to detect hepatitis C. It was an ideal reagent to detect hepatitis C.
In order to improve the diagnostic efficiency of the hepatitis C in China, the DNA fragments of core, C33c,NS4 in Hepatitis C virus(HCV) were connected and subcloned to pET24a(+). The pET24a-CCN was highly expressed with the inducement IPTG in the E.coli BL21(DE3). In 1L bacterial culture medium,30mg purified recombinant proteins were available using His Binding Resin under denatured condition. Used as diagnostic antigen to detect the human hepatitis C by ELISA, The results showed that the 100% positive coincidence rate (66/66) and 94.5% negative coincidence rate (86/91) were obtained. To detect 46 positive HAV sera and 46 positive HBs sera, all the results were negative. At the same time, the freeze-dried recombinant antigen detects 66 HCV positive sera, all the results were positive. Therefore, the recombinant antigen was sensitivity, specialty and stability to detect hepatitis C. It was an ideal reagent to detect hepatitis C.
出处
《中国病毒学》
CSCD
2005年第2期206-208,共3页
Virologica Sinica
基金
上海市科技兴农重点攻关项目农科攻字(2000)第5 08号
