摘要
利用制备高效液相色谱法从喜树细胞中分离制备了在原植株内不存在的两种全新的生物碱单体喜树异碱A和喜树异碱B。在C18柱上,以乙腈水(体积比为35∶65)为流动相,流速为5mL/min,采用等度洗脱方式,制备了这两种化合物。经紫外光谱、红外光谱及质谱分析,对这两种化合物进行了确认。高效液相色谱分析表明,制备的喜树异碱A和喜树异碱B的纯度均在97% (质量分数)以上。在该色谱条件下,各色谱峰达到了基线分离,且分离时间短,溶剂用量少。
A method using preparative high performance liquid chromatography ( HPLC) to isolate two individial alkaloids from cells of Camptotheca acuminata Decaisne was investigated. A preparative C-18 column was employed in the procedure. By isocratic elution with acetonitrile-water (35:65, V/V) as mobile phase at a flow rate of 5 mL/min, two alkaloids were obtained and subsequently were indetified by ultraviolet, infrared and mass spectroscopic methods as isocamptothecin A and isocamptothecin B. HPLC analytical results showed that the purity of each alkaloid was over 97%. Under the chromatographic conditions, every peak was completely baseline separated and consumption of solvents was low.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第4期501-504,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No. 20176058)
