摘要
以斑带石斑鱼与黑边石斑鱼为材料，胸腔注射秋水仙碱溶液，取全肾经空气干燥制片法制片。姬姆萨染色和银染，分析核型及Ａｇ－ＮＯＲ带，结果表明斑带石斑鱼的核型为２ｎ＝４８、４８ｔ、ＮＦ＝４８。黑边石斑鱼的核型为２ｎ＝４８、４８ｔ、ＮＦ＝４８。在最小一对端部着丝粒染色体上均具有明显的次缢痕。银染结果显示该次缢痕部位即为核仁组织者区域。
The metaphase chromosome specimens were prepared from kindney cell injected colchicine. Air-drying technique, Giemsa stain and Ag-Nucleolus were used.The results show the diploid chromosome number of E. fasciatomaculatus or E. fasciams is 48.There is a pair of chromosomes with secondary constriction in t group.
关键词
斑带石斑鱼
黑边石斑鱼
核型
次缢痕
Erinephelus fasciatomaculatus
Epinephelus fasciatus
Karyotype
Secondary constriCtion
Ag-Nucleolus organizer chromosome
