摘要
研究和评价了蝇蛆几丁低聚糖咀嚼片的调节血脂作用及其安全性。将50只实验动物 (大鼠) 随机分为普通饲料对照组、高脂饲料对照组和3个实验组, 实验组分别喂以不同剂量蝇蛆几丁低聚糖咀嚼片和高脂饲料。饲养28天后, 分别测定大鼠血清总胆固醇 (total cholesterol, TC)、血清总甘油三酯 (triglyceride, TG)、血清高密度脂蛋白胆固醇 (high densitylipoprotein cholesterol, HDLC) 的含量。用Horn’s法对蝇蛆几丁低聚糖咀嚼片的急性毒性进行研究, 连续观察7天, 记录各组动物的中毒反应情况和死亡只数, 计算咀嚼片对小鼠的半数致死剂量。同时对几丁低聚糖咀嚼片作了调节血脂的动物试验, 研究结果表明, 蝇蛆几丁低聚糖咀嚼片对大鼠血清总胆固醇、总甘油三酯有明显降低和对血清高密度脂蛋白胆固醇有明显稳定作用, 具有辅助降血脂作用; 对咀嚼片进行急性毒性实验表明, 蝇蛆几丁低聚糖咀嚼片对小鼠的半数致死剂量大于10 g kg·bw, 提示其基本无毒。
To evaluate the effects of lowering hyperlipemia of the chewing tablets of oligochitosan from housefly larvae and assess its security, 50 rats were divided into 5 experimental groups. One control group was fed with common feed, another control group was fed with high fattiness feed, and three treatment groups were fed with different dosage of tablets of oligochitosan from housefly larvae and high fattiness feed, respectively. After 28 days, the content of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDLC) in the serum of rats were measured. The acute toxicity of the chewing tablets of oligochitosan was studied according to the guidelines of Chinese Traditional Medicine Pre clinical Trial. The toxicosis reaction and death number of the mouse were recorded successively for 7 days, and then its LD (50) were calculated accordingly. The results showed that when rats were treated with the chewing tablets of oligochitosan, the contents of TC, TG in rat serum decreased remarkably but HDLC increased significantly. The results suggest that the chewing tablets of oligochitosan from housefly larvae may assist in lowering hyperlipemia. The result of acute toxicity study showed its LD (50) exceeded 10 g/kg·bw, which indicated that the chewing tablets was a safe product.
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第2期314-318,共5页
Acta Entomologica Sinica
