摘要
目的 探讨采用蜕膜建立人子宫内膜异位症移植动物模型的可行性。方法 将早孕期蜕膜组织种植于NOD- SCID鼠腹部皮下,随机分成4组,分别在2、4、6、8周,取出其皮下移植物送病理检查,并用免疫组织化学的方法观察其形态学、增殖活性方面有无改变。结果 16只小鼠均存活,有15只小鼠皮下病灶经HE染色可见明显的子宫内膜腺体和间质,成功率为93. 75%。腺上皮细胞角蛋白、ki67染色均阳性,基质细胞波形蛋白染色阳性, 8周移植物中一些基质细胞减少区域的腺上皮细胞波形蛋白染色阳性。结论 采用人蜕膜建立NOD- SCID小鼠子宫内膜异位症模型成功率高,模型形状显著且稳定。
Objective To establish the implanted animal model of human endometriosis with deciduas. Methods The decidua of early pregnant was implanted subcutaneously in the abdomen of NOD-SCID mice.The mice were randomly divided into four groups. The implant was saved for pathology examination at 2nd,4th,6th,8th weeks. Results Successful subcutaneous implantation was observed in 15 of 16 (93.75%) NOD-SCID mice. Haematoxylin-eosin staining of implanting lesions demonstrated the presence of endometrial glandular tissue in a background of stromal cells. The glandular epithelium exhibited positive cytokeratin and ki67 staining.The stromal cells exhibited positive vimentin staining. The glandular epithelium exhibited positive vimentin staining in combination with a decrease of surrounding stromal cells at 8th week. Conclusion The establishment of the animal model of endometriosis with deciduas is successful.
出处
《安徽医科大学学报》
CAS
北大核心
2005年第2期99-101,共3页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金资助项目(编号: 30471805)
