摘要
目的:对小鼠尾静脉单次推注普鲁泊福后,观察其血清中普鲁泊福质量浓度和脑组织中普鲁泊福质量比的变化,测定脑组织中的普鲁泊福消除半衰期。方法:选择小鼠 140只,以 32mg/kg的剂量,单次推注普鲁泊福。按照给药后 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 30, 45和 60min,分为 14组,每组 10只,摘取眼球,取动脉血,断头取全脑。采用高效液相色谱检测法测定普鲁泊福含量,并计算脑组织的消除半衰期。结果:小鼠静脉单次推注普鲁泊福后血清的达峰时间为 1min,脑组织的达峰时间为 3min。脑组织的消除半衰期为(9. 6±0. 5)min。结论:脑组织中的峰浓度滞后于血清中的峰浓度。脑组织的消除半衰期能更直接地反应普鲁泊福在效应室的消除状态。
Objective: To study the elimination half-life of propofol in mouse brain. Methods: One hundred and forty mice were injected with propofol 32 mg/kg through caudal vein. The mice were divided into 14 groups with different time points, i.e. 1,2,3,4,5,6,7,8,9,10,15,30,45,and 60 min,with 10 mice in each group. The mice were killed to obtain arterial blood by picking off their eyeballs, and their brains by cutting off their heads. The concentrations of propofol in serum and brain were measured by high performance liquid chromatography. The parameters of elimination half-life were calculated by software 3P97. Results: Time to peak concentration of propofol in serum was about 1 min, and time to peak concentration of propofol in brain was about 3 min. The elimination half-life of propofol in brain were (9.6± 0.5) mins. Conclusion: The peak concentrations of propofol in brain was later than it in serum. There is correlation between the concentrations of propofol in blood and in brain. The metabolism of propofol in effect site could be measured accurately by the elimination half-life in brain.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2005年第2期187-189,共3页
Journal of Peking University:Health Sciences
