摘要
目的探讨缝隙连接在脑血管痉挛中的作用及观察其阻断剂在动物实验中的治疗作用。方法建立兔二次蛛网膜下腔出血模型,通过脑血管造影观察经动脉或池内注入缝隙连接阻断剂heptanol,对脑血管痉挛的抑制和治疗作用,并观察基底动脉的形态学变化。结果枕大池注血后血管造影显示基底动脉出现痉挛。在脑血管痉挛后,动脉给予heptanol对急、慢性脑血管痉挛有显著的治疗作用。预先枕大池注入heptanol再注血,造影显示基底动脉痉挛不明显(P>0.05),heptanol枕大池预处理后能显著抑制急、慢性期脑血管痉挛的形成。形态学检查发现,对照组第7d的基底动脉光镜见内皮细胞核染色质聚集,内弹力膜波纹状,平滑肌细胞分布稀疏等。动脉给药组和池内给药组也有类似变化,但范围局限、程度轻。结论缝隙连接阻断剂heptanol能有效抑制兔蛛网膜下腔出血后急性和慢性脑血管痉挛,体内试验表明缝隙连接在脑血管痉挛中可能发挥重要作用,应用其阻断剂heptanol具有显著的治疗作用。
Objective To investigate the role of gap junctions and observe the therapeutic efficacy of heptanol, a gap junctional blocker, in the experimental cerebral vasospasm. Methods The rabbit two-hemorrhage model was established by injection of autogenous blood into the cisterna magna and the diameters of the basilar arteries were determined through arteriography. The therapeutic efficacy of heptanol given intraarterially and intracisternally respectively was observed. Light microscope was performed to assess the morphologic changes of the arteries examined.Results Vasospasm of basilar arterial occurred after injection of the autogenous blood into the cisterna magna. In the acute phase, basilar arterial vasospasm relieved 10 minutes after intraarterial infusion of 0.2 mmol/kg heptanol. In the chronic phase, the diameters of the arteries on day 7 did not significantly changed as compared to those of the arteries in control groups after the second injectons of blood and heptanol on day 2. The results demonstrated the therapeutic efficacy of heptanol in the acute and chronic cerebral vasospasm. When heptanol was previously injected intracistrnally 10 minutes before blood injection , the cerebral vasospasm was not obvious. The results showed that heptanol could pre-inhibit the formation of the acute and chronic cerebral vasospasm . Through light microscope, the basilar arteries from day 7 rabbits of the control group had abnormalities including condensation of chromatin in endothelial cells, corrugation of internal elastic lamina, sparse distributing of smooth muscle cells, etc. The vessels of the intraarterial and intracisternal treatment group demonstrated less extensive degenerative changes.Conclusion Heptanol can inhibit the acute and chronic rabbit basilar arterial vasospasm after subarachnoid hemorrhage . The gap junctions may play a pathophysiological role in the cerebral vasospasm. And heptanol, the gap junction blocker, has a therapeutic efficacy in the experimental cerebral vasospasm.
出处
《中华神经外科杂志》
CSCD
北大核心
2005年第4期244-247,共4页
Chinese Journal of Neurosurgery
基金
国家自然科学基金资助项目(39960076)
