摘要
目的 寻求建立慢性阻塞性肺疾病(COPD)大鼠模型的新方法。方法 以熏烟、寒冷刺激4周,结合两次气管内注入脂多糖的多因素刺激制作COPD大鼠模型。结果 COPD模型组大鼠体重增幅下降(P<0 .0 1) ,呼吸频率高于正常对照组(P <0 .0 1) ,而温水游泳时间则低于正常对照组(P <0 .0 1) ,0 .3s用力呼气容积占用力肺活量比、呼气峰值流速、呼气中期流速较正常对照组降低(P <0 .0 1) ,吸气阻力、呼气阻力,较正常对照组增高(P <0 .0 1) ;COPD模型组大鼠气管、支气管及肺组织有慢性炎症及阻塞性肺气肿的特征性病理改变。结论 采用熏烟、寒冷和脂多糖多因素复合刺激的方法,可成功制备COPD大鼠模型,该模型不仅病理形态学和肺功能的改变与人类COPD类似,而且在整体状况和运动能力方面的变化与人类COPD的临床病程和表现更接近。
Objective To find a way of es ta blishing a multifactorial model of chronic obstructive pulmonary disease in rat. Methods The COPD model was established by inhalation cigarette, exposure to cold and intratracheal instillation of lipopolysaccharide.Re sults In model group,amplification of weight reduced(P<0.01),res piratory frequency increased(P<0.01),and the swimming time in warm water decreased(P<0.01) than that of control group.Percentage of forced expirat ory volume in first 0.3 second to forced vital capacity (FEV0.3/FVC%),peak expir atory flow (PEF),maxium midexpiratory flow (MMF) and dynamic lung compliance (CL dyn) in model group were significant lower in comparison with those of control g roup(P<0.01),while inspiratory resisstance(Ri) and expiratory resisstanc e (Re) increased significantly(P<0.01).The model group rats shared specif ic pathological features in trachea,bronchi and lung tissues with that of human chronic airway inflammation and obstructive emphesema.Conclusions By inhaling cigarette and exposuring to cold and intratrachealy lipopolysaccharide,the COPD rat model is successfully established and share man y characteristics of human COPD,not only in the pathological,lung function and a lso in total condition and locomotivity.
出处
《中国临床保健杂志》
CAS
2005年第2期145-147,共3页
Chinese Journal of Clinical Healthcare
关键词
肺疾病
慢性阻塞性
大鼠
模型
动物
Pulmona ry disease,chronic obstructive
Rats
Models,animal
