摘要
以广西、广东、湖南、贵州、四川、湖北、江苏、安徽、山东、河南等10省共31个地理种源为材料,对其PCR各种反应条件实验进行了研究。结果表明: PCR反应时,总反应液20μL中基因组DNA浓度为20ng,引物浓度为10μmol/L,dNTP浓度为200μmol/L,镁离子的浓度选择1. 5mmol/L,退火温度为38℃下出现可辨认的鲜明的谱带。本研究为RAPD分析应用于何首乌遗传多样性研究和优良种源选择打下了良好的基础。
The effects of several paramenters on PCR amplification of Polygonum multilorum Thunb. were studied. The results showed that the bands were clear in the following conditions: Approximately the genomic DNA volume was 20 ng and that of primers was 10 pd; primer concentration was 200 μmol/L; dNTP concentration was 200 μmol/L; Mg^(2+) concentration was 2.0 mmol/L; annealing temperature was 38 ℃.The RAPD analysis procedure in this experiment lays a good foundation for application of RAPD in Polygonum multilorum Thunb.genetic research and selection and breeding of fine-breeds.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2005年第2期265-269,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
江苏省高科技研究项目(BG2004314)
关键词
何首乌
DNA
提取
RAPD
Polygonum multilorum Thunb.
DNA
extraction
RAPD
