摘要
目的:测定生发灵酊(当归、红花、何首乌等)中阿魏酸和大黄酸的含量.方法:高效毛细管电泳法.毛细管(60cm×75μm,有效长度53cm);运行缓冲液30mmol·L-1硼砂(pH 8.2),高压进样5s,分离电压12kV,温度为25℃,检测波长为313nm,咖啡酸为内标.结果:阿魏酸在2.4～12μg·mL-1浓度范围内线性关系良好(r=0.9997),平均回收率为99.06%.大黄酸在1.6～8μg·mL-1浓度范围内线性关系良好(r=0.999 6),平均回收率为98.94%.结论:本法简单、快捷、灵敏.
AIM: To determine the content of ferulic acid and rhein in Shengfaling Tincture(Radix Angelicae Sinensis,Flos Carthami,Radix Polygoni Multiflori,ect.). METHODS:HPCE was used.The best condition was obtained with a fuse dsilca capillary tube (60 cm×75 μm,effective length of 53 cm),30 mmol·L -1 sodium tetraborate electrophoretic buffer(pH 8.2),at a constant voltage of 12 kV and temperature at 25 °C .The UV detection wavelength was at 313 nm and caffeic acid was adopted as internal standard. RESULTS:The calibration curves was linear in the range of 2.4-12 μg·mL -1 for ferulic acid(r=0.999 7) and 1.6-8 μg·mL -1 for rhein (r= 0.999 6 ),respectively.The average recovery of ferulic acid was 99.06% and that of rhein was 98.94%. CONCLUSION: This method is simple,quick and sensitive.
出处
《中成药》
CAS
CSCD
北大核心
2005年第4期402-404,共3页
Chinese Traditional Patent Medicine
